Cell Signaling Technology

Product Pathways - Innate Immunity

DDX41 (D3F1Z) Rabbit mAb #15076

No. Size Price
15076S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15076 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 75 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

DDX41 (D3F1Z) Rabbit mAb recognizes endogenous levels of total DDX41 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala98 of human DDX41 protein.

IP

IP

Immunoprecipitation of DDX41 from THP-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is DDX41 (D3F1Z) Rabbit mAb. Western blot analysis was performed using DDX41 (D3F1Z) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DDX41 (D3F1Z) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human DDX41 protein (hDDX41-Myc/DDK; +), using DDX41 (D3F1Z) Rabbit mAb.

Background

The DEAD box helicase DDX41 is an antiviral protein that senses cytosolic DNA and activates the signaling adaptor STING to induce type I interferon production (1).  The recognition of bacterial dinucleotides by DDX41 also leads to STING-dependent type 1 interferon production (2).  Research studies demonstrate that knockdown of DDX41 attenuates poly(dA:dT)-induced IFN-β production and cyclic GMP-AMP synthase (cGAS, MB21D1) induction (3). The DDX41 protein is thought to be an initial constitutively expressed DNA sensor that activates type I interferon production and leads to cGAS amplification of type I interferon production (3). DDX41 is negatively regulated by the E3 ligase TRIM21 (4). Both germline and somatic mutations in the corresponding DDX41 gene are associated with myeloid neoplasms (5,6). 

  1. Zhang, Z. et al. (2011) Nat Immunol 12, 959-65.
  2. Parvatiyar, K. et al. (2012) Nat Immunol 13, 1155-61.
  3. Ma, F. et al. (2015) J Immunol 194, 1545-54.
  4. Zhang, Z. et al. (2013) Nat Immunol 14, 172-8.
  5. Ding, L. et al. (2012) Nature 481, 506-10.
  6. Polprasert, C. et al. (2015) Cancer Cell 27, 658-70.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications