Cell Signaling Technology

Product Pathways - Apoptosis

Bcl-2 (124) Mouse mAb #15071

sc-7382  

No. Size Price
15071S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15071T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
15071 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 26 Mouse IgG1
IP 1:50
IHC-P 1:400
F 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,

Specificity / Sensitivity

Bcl-2 (124) Mouse mAb recognizes endogenous levels of total Bcl-2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly47 of human Bcl-2 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded RL-7 (positive, left) and HT-29 (negative, right) cell pellets using Bcl-2 (124) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using Bcl-2 (124) Mouse mAb.

IP

IP

Immunoprecipitation of Bcl-2 from RL-7 cell extracts using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or Bcl-2 (124) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Bcl-2 (D55G8) Rabbit mAb (Human Specific) #4223.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Bcl-2 (124) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). KARPAS cell Line source: Dr Abraham Karpas at the University of Cambridge.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Bcl-2 (124) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Bcl-2 (124) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HT29 cells (blue), SHSY5Y cells (red) and RL7 cells (green) using Bcl-2 Mouse mAb. Anti-mouse IgG (H+L), F(ab')2 fragment (Alexa Fluor 488 Conjugate) #4412 was used as a secondary antibody.

Background

Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74, and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).

  1. Murphy, K.M. et al. (2000) Cell Death Differ 7, 102-11.
  2. Zhu, L. et al. (1999) J Biol Chem 274, 33267-73.
  3. Maundrell, K. et al. (1997) J Biol Chem 272, 25238-42.
  4. Yamamoto, K. et al. (1999) Mol Cell Biol 19, 8469-78.
  5. Ling, Y.H. et al. (1998) J Biol Chem 273, 18984-91.
  6. Huang, S.T. and Cidlowski, J.A. (2002) FASEB J 16, 825-32.
  7. Deng, X. et al. (2001) J Biol Chem 276, 23681-8.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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