Product Pathways - Tag Antibodies
Cre Recombinase (D7L7L) XP® Rabbit mAb #15036
|15036S||100 µl ( 10 western blots )||￥3,580.00 现货查询||购买询价|
|15036T||20 µl ( 2 western blots )||￥1,400.00 现货查询||购买询价|
|15036||carrier free & custom formulation / quantity||email request|
|W||1:1000||All Species Expected,||Recombinant||37||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),
Specificity / Sensitivity
Cre Recombinase (D7L7L) Rabbit mAb recognizes transfected and transgenic levels of total Cre recombinase protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of bacteriophage-P1 Cre recombinase protein.
Immunohistochemical analysis of paraffin-embedded Villin-CreERT2 mouse small intestine (left) and wild type mouse small intestine (right) using Cre Recombinase (D7L7L) XP® Rabbit mAb. Villin-CreERT2 mouse small intestine kindly provided by Omer Yilmaz of MIT, Cambridge, MA.
Immunohistochemical analysis of paraffin-embedded 293T cell pellets, untransfected (left) or transfected with Cre recombinase (right), using Cre Recombinase (D7L7L) XP® Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock-transfected (-) or transfected with a construct expressing Cre recombinase (+), using Cre Recombinase (D7L7L) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded Lgr5-EGFP-IRES-CreERT2 mouse small intestine using Cre Recombinase (D7L7L) XP® Rabbit mAb. Lgr5-EGFP-IRES-CreERT2 mouse small intestine kindly provided by Omer Yilmaz of MIT, Cambridge, MA.
Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34 base pair loxP sites. This reaction can be carried out in vitro, indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of in vitro and in vivo applications including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6).
- Abremski, K. et al. (1983) Cell 32, 1301-11.
- Sternberg, N. et al. (1981) Cold Spring Harb Symp Quant Biol 45 Pt 1, 297-309.
- Abremski, K. and Hoess, R. (1984) J Biol Chem 259, 1509-14.
- Qin, M. et al. (1994) Proc Natl Acad Sci U S A 91, 1706-10.
- Lakso, M. et al. (1992) Proc Natl Acad Sci U S A 89, 6232-6.
- Guo, F. et al. (1997) Nature 389, 40-6.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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