Cell Signaling Technology

Product Pathways - Tag Antibodies

Cre Recombinase (D7L7L) XP® Rabbit mAb #15036

No. Size Price
15036S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
15036T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
15036 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Recombinant 37 Rabbit IgG
IHC-P 1:125
F 1:50
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Cre Recombinase (D7L7L) Rabbit mAb recognizes transfected and transgenic levels of total Cre recombinase protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of bacteriophage-P1 Cre recombinase protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Villin-CreERT2 mouse small intestine (left) and wild type mouse small intestine (right) using Cre Recombinase (D7L7L) XP® Rabbit mAb. Villin-CreERT2 mouse small intestine kindly provided by Omer Yilmaz of MIT, Cambridge, MA.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded 293T cell pellets, untransfected (left) or transfected with Cre recombinase (right), using Cre Recombinase (D7L7L) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock-transfected (-) or transfected with a construct expressing Cre recombinase (+), using Cre Recombinase (D7L7L) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Lgr5-EGFP-IRES-CreERT2 mouse small intestine using Cre Recombinase (D7L7L) XP® Rabbit mAb. Lgr5-EGFP-IRES-CreERT2 mouse small intestine kindly provided by Omer Yilmaz of MIT, Cambridge, MA.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells transfected with a construct expressing Cre Recombinase using Cre Recombinase (D7L7L) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 650 Phalloidin (red pseudocolor).

Background

Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34 base pair loxP sites. This reaction can be carried out in vitro, indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of in vitro and in vivo applications including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6).

  1. Abremski, K. et al. (1983) Cell 32, 1301-11.
  2. Sternberg, N. et al. (1981) Cold Spring Harb Symp Quant Biol 45 Pt 1, 297-309.
  3. Abremski, K. and Hoess, R. (1984) J Biol Chem 259, 1509-14.
  4. Qin, M. et al. (1994) Proc Natl Acad Sci U S A 91, 1706-10.
  5. Lakso, M. et al. (1992) Proc Natl Acad Sci U S A 89, 6232-6.
  6. Guo, F. et al. (1997) Nature 389, 40-6.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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