Cell Signaling Technology

Product Pathways - Neuroscience

Neurofascin 155 (D7B6O) Rabbit mAb #15035

No. Size Price
15035S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15035T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
15035 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 140-155 Rabbit IgG
IF-F 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

Neurofascin 155 (D7B6O) recognizes endogenous levels of total neurofascin 155 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg881 of human neurofascin 155 protein.

IF-F

IF-F

Confocal immunofluorescent analysis of rat spinal cord using Neurofascin 186 (D6G6O) Rabbit mAb (left, green) or Neurofascin 155 (D7B6O) Rabbit mAb # 15035 (right, green). β3-Tubulin protein was labeled with β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, mouse lung, and rat brain using Neurofascin 155 (D7B6O) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IF-F

IF-F

Confocal immunofluorescent analysis of mouse sciatic nerve using Neurofascin 186 (D6G6O) Rabbit mAb (left, green) or Neurofascin 155 (D7B6O) Rabbit mAb # 15035 (right, green). Neurofilament-L protein was labeled with Neurofilament-L (DA2) Mouse mAb #2835 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Myelinated axons contain un-myelinated gaps called nodes of Ranvier. These regularly spaced gaps are critical for the proper propagation and rapid conduction of nerve impulses in the central and peripheral nervous system (1). The structure and organization of the nodes of Ranvier is dictated by interaction between the axon and glial cells (2). Voltage-gated sodium channels concentrated at the nodes and potassium channels clustered at the paranodes are responsible for propagation of the action potentials (3,4). Other proteins that contribute to the architecture and function of the nodes of Ranvier include βIV spectrin (5), ankyrin-G (6), and the L1 cell adhesion molecules, neurofascin and NrCAM (7,8).

Alternative splicing produces several neurofascin isoforms that differ in temporal and spatial expression. Neurofascin 186 is expressed in axons where it is concentrated at the nodes. Research studies indicate that neurofascin 186 is responsible for nodal assembly and clustering of sodium channels (9). Neurofascin 155 is expressed in glial cells and is localized to myelin paranodes. Interactions between neurofascin 155 and the contactin-associated protein (Caspr) tether the myelin sheath to the axon (10). N-linked glycosylation results in two forms of neurofascin 155 (high and low) that are differentially expressed during development (11).

  1. Black, J.A. et al. (1990) Trends Neurosci 13, 48-54.
  2. Salzer, J.L. (1997) Neuron 18, 843-6.
  3. Waxman, S.G. et al. (1989) Proc Natl Acad Sci U S A 86, 1406-10.
  4. Ritchie, J.M. (1992) Trends Neurosci 15, 345-51.
  5. Berghs, S. et al. (2000) J Cell Biol 151, 985-1002.
  6. Zhou, D. et al. (1998) J Cell Biol 143, 1295-304.
  7. Davis, J.Q. et al. (1996) J Cell Biol 135, 1355-67.
  8. Ratcliffe, C.F. et al. (2001) J Cell Biol 154, 427-34.
  9. Thaxton, C. et al. (2011) Neuron 69, 244-57.
  10. Charles, P. et al. (2002) Curr Biol 12, 217-20.
  11. Pomicter, A.D. et al. (2010) Brain 133, 389-405.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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