Product Pathways - Neuroscience
Neurofascin 186 (D6G6O) Rabbit mAb #15034
|15034S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|15034||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen),
Specificity / Sensitivity
Neurofascin 186 (D6G6O) Rabbit mAb recognizes endogenous levels of total neurofascin 186 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr1108 of human neurofascin 186 protein.
Confocal immunofluorescent analysis of rat spinal cord using Neurofascin 186 (D6G6O) Rabbit mAb (left, green) or Neurofascin 155 (D7B6O) Rabbit mAb # 15035 (right, green). β3-Tubulin protein was labeled with β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Western blot analysis of extracts from mouse brain, mouse lung, and rat brain using Neurofascin 186 (D6G6O) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of Neurofascin 186 from mouse brain extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Neurofascin 186 (D6G6O) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Neurofascin 186 (D6G6O) Rabbit mAb.
Confocal immunofluorescent analysis of mouse sciatic nerve using Neurofascin 186 (D6G6O) Rabbit mAb (left, green) or Neurofascin 155 (D7B6O) Rabbit mAb # 15035 (right, green). Neurofilament-L protein was labeled with Neurofilament-L (DA2) Mouse mAb #2835 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Myelinated axons contain un-myelinated gaps called nodes of Ranvier. These regularly spaced gaps are critical for the proper propagation and rapid conduction of nerve impulses in the central and peripheral nervous system (1). The structure and organization of the nodes of Ranvier is dictated by interaction between the axon and glial cells (2). Voltage-gated sodium channels concentrated at the nodes and potassium channels clustered at the paranodes are responsible for propagation of the action potentials (3,4). Other proteins that contribute to the architecture and function of the nodes of Ranvier include βIV spectrin (5), ankyrin-G (6), and the L1 cell adhesion molecules, neurofascin and NrCAM (7,8).
Alternative splicing produces several neurofascin isoforms that differ in temporal and spatial expression. Neurofascin 186 is expressed in axons where it is concentrated at the nodes. Research studies indicate that neurofascin 186 is responsible for nodal assembly and clustering of sodium channels (9). Neurofascin 155 is expressed in glial cells and is localized to myelin paranodes. Interactions between neurofascin 155 and the contactin-associated protein (Caspr) tether the myelin sheath to the axon (10). N-linked glycosylation results in two forms of neurofascin 155 (high and low) that are differentially expressed during development (11).
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- Waxman, S.G. et al. (1989) Proc Natl Acad Sci U S A 86, 1406-10.
- Ritchie, J.M. (1992) Trends Neurosci 15, 345-51.
- Berghs, S. et al. (2000) J Cell Biol 151, 985-1002.
- Zhou, D. et al. (1998) J Cell Biol 143, 1295-304.
- Davis, J.Q. et al. (1996) J Cell Biol 135, 1355-67.
- Ratcliffe, C.F. et al. (2001) J Cell Biol 154, 427-34.
- Thaxton, C. et al. (2011) Neuron 69, 244-57.
- Charles, P. et al. (2002) Curr Biol 12, 217-20.
- Pomicter, A.D. et al. (2010) Brain 133, 389-405.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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