Cell Signaling Technology

Product Pathways - DNA Damage

Rad54 (D4W3Z) Rabbit mAb #15016

No. Size Price
15016S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
15016 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 84 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Rad54 (D4W3Z) Rabbit mAb recognizes endogenous levels of total Rad54 protein. Based on sequence, this antibody is not expected to recognize Rad54B. This antibody also cross-reacts with an unidentified protein of 200 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly246 of human Rad54 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Rad54 (D4W3Z) Rabbit mAb.



Immunoprecipitation of Rad54 from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Rad54 (D4W3Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Rad54 (D4W3Z) Rabbit mAb.


DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells sense and repair DSBs via two distinct but partly overlapping signaling pathways, nonhomologous end joining (NHEJ) and homologous recombination (HR). Defects in both pathways have been associated with human disease, including cancer (1). The DNA repair and recombination protein RAD54-like (Rad54, RAD54L) is a Swi2/Snf2 family DNA helicase that is involved in homologous recombination DNA repair. Rad54 is a double-stranded DNA-dependent ATPase that translocates in a processive manner along double-stranded DNA. The Rad54 helicase interacts with the Rad51 recombinase to regulate its DNA binding and strand exchange activities during homologous recombination (2-4). Mutations in the corresponding RAD54L gene are associated with multiple forms of human cancer, including non-Hodgkin's lymphoma, breast cancer, and parathyroid adenoma (5-8).

  1. Hartlerode, A.J. and Scully, R. (2009) Biochem J 423, 157-68.
  2. Sung, P. et al. (2003) J Biol Chem 278, 42729-32.
  3. Wright, W.D. and Heyer, W.D. (2014) Mol Cell 53, 420-32.
  4. Ceballos, S.J. and Heyer, W.D. (2011) Biochim Biophys Acta 1809, 509-23.
  5. Smirnova, M. et al. (2004) J Biol Chem 279, 24081-8.
  6. Gonzalez, R. et al. (1999) Br J Cancer 81, 503-9.
  7. Carling, T. et al. (1999) Int J Cancer 83, 80-2.
  8. Matsuda, M. et al. (1999) Oncogene 18, 3427-30.

Application References

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