Product Pathways - Metabolism
MTHFD1L (D8T7L) Rabbit mAb #14999
|14999S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|14999||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting,
Specificity / Sensitivity
MTHFD1L (D8T7L) Rabbit mAb recognizes endogenous levels of total MTHFD1L protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala264 of human MTHFD1L protein.
NADP+ dependent methylenetetrahydrofolate dehydrogenase 1-like (MTHFD1L) is a mitochondrial enzyme that catalyzes the production of formate from 10-formyl-tetrahydrofolate, the last step in one-carbon (1-C) flow from mitochondria to cytoplasm (1,2). These one-carbon end products are required for de novo synthesis of thymidylate and purines. In the mitochondria, these essential one-carbon products are formed by a series of reactions catalyzed by a pair of enzymes (MTHFD2 and MTHFD1L), but by the trifunctional MTHFD1 enzyme in the cytoplasm (3). The 10-formyl-tetrahydrofolate synthetase MTHFD1L is widely expressed in most adult tissues and at all stages of mammalian embryonic development (1). Research studies using MTHFD1L knockout mice indicate that MTHFD1L plays an essential role in neural tube formation; mice lacking MTHFD1L displayed neural tube and craniofacial defects leading to embryonic lethality (4).
- Prasannan, P. et al. (2003) J Biol Chem 278, 43178-87.
- Prasannan, P. and Appling, D.R. (2009) Arch Biochem Biophys 481, 86-93.
- Pike, S.T. et al. (2010) J Biol Chem 285, 4612-20.
- Momb, J. et al. (2013) Proc Natl Acad Sci U S A 110, 549-54.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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