Cell Signaling Technology

Product Pathways - Metabolism

MTHFD1L (D7O8E) Rabbit mAb #14998

No. Size Price
14998S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14998 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 106 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

MTHFD1L (D7O8E) Rabbit mAb recognizes endogenous levels of total MTHFD1L protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro52 of human MTHFD1L protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MTHFD1L (D7O8E) Rabbit mAb.

IP

IP

Immunoprecipitation of MTHFD1L from 293 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MTHFD1L (D7O8E) Rabbit mAb. Western blot analysis was performed using MTHFD1L (D7O8E) Rabbit mAb.

Background

NADP+ dependent methylenetetrahydrofolate dehydrogenase 1-like (MTHFD1L) is a mitochondrial enzyme that catalyzes the production of formate from 10-formyl-tetrahydrofolate, the last step in one-carbon (1-C) flow from mitochondria to cytoplasm (1,2). These one-carbon end products are required for de novo synthesis of thymidylate and purines. In the mitochondria, these essential one-carbon products are formed by a series of reactions catalyzed by a pair of enzymes (MTHFD2 and MTHFD1L), but by the trifunctional MTHFD1 enzyme in the cytoplasm (3). The 10-formyl-tetrahydrofolate synthetase MTHFD1L is widely expressed in most adult tissues and at all stages of mammalian embryonic development (1). Research studies using MTHFD1L knockout mice indicate that MTHFD1L plays an essential role in neural tube formation; mice lacking MTHFD1L displayed neural tube and craniofacial defects leading to embryonic lethality (4).

  1. Prasannan, P. et al. (2003) J Biol Chem 278, 43178-87.
  2. Prasannan, P. and Appling, D.R. (2009) Arch Biochem Biophys 481, 86-93.
  3. Pike, S.T. et al. (2010) J Biol Chem 285, 4612-20.
  4. Momb, J. et al. (2013) Proc Natl Acad Sci U S A 110, 549-54.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications