Cell Signaling Technology

Product Pathways - Apoptosis

MLKL (D2I6N) Rabbit mAb #14993

No. Size Price
14993S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14993 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 54 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

MLKL (D2I6N) Rabbit mAb recognizes endogenous levels of total MLKL protein. This antibody also cross-reacts with an unidentified protein of 130 kDa in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLKL protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MLKL (D2I6N) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human MLKL protein (hMLKL-Myc/DDK; +), using MLKL (D2I6N) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).


Necroptosis, a regulated pathway for necrotic cell death, is triggered by a number of inflammatory signals including cytokines in the tumor necrosis factor (TNF) family, pathogen sensors such as toll-like receptors (TLRs), and ischemic injury (1,2). The process is negatively regulated by caspases and is initiated through a complex containing the RIP1 and RIP3 kinases, typically referred to as the necrosome. Mixed lineage kinase domain-like protein (MLKL) is a pseudokinase that was identified as downstream target of RIP3 in the necroptosis pathway (3,4). During necroptosis RIP3 is phosphorylated at Ser227, which recruits MLKL and leads to its phosphorylation at Thr357 and Ser358 (3). Knockdown of MLKL through multiple mechanisms results in inhibition of necroptosis (3-5). While the precise mechanism for MLKL-induced necroptosis is unclear, some studies have shown that necroptosis leads to oligomerization of MLKL and translocation to the plasma membrane, where it effects membrane integrity (6-9).

  1. Christofferson, D.E. and Yuan, J. (2010) Curr Opin Cell Biol 22, 263-8.
  2. Kaczmarek, A. et al. (2013) Immunity 38, 209-23.
  3. Sun, L. et al. (2012) Cell 148, 213-27.
  4. Wang, Z. et al. (2012) Cell 148, 228-43.
  5. Wu, J. et al. (2013) Cell Res 23, 994-1006.
  6. Cai, Z. et al. (2014) Nat Cell Biol 16, 55-65.
  7. Chen, X. et al. (2014) Cell Res 24, 105-21.
  8. Wang, H. et al. (2014) Mol Cell 54, 133-46.
  9. Dondelinger, Y. et al. (2014) Cell Rep 7, 971-81.

Application References

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