Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

OX40L (D6X2D) Rabbit mAb #14991

CD134L   CD252   OX-40L   OX40L   TNFSF4  

No. Size Price
14991S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14991 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 30 Rabbit IgG
IP 1:50
F 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry,

Specificity / Sensitivity

OX40L (D6X2D) Rabbit mAb recognizes endogenous levels of total OX40L protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human OX40L protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (left) or treated (right), using OX40L (D6X2D) Rabbit mAb and co-stained with anti-human CD14 antibody. Cells were treated with Human Interleukin-4 (hIL-4) #8919 (20 ng/ml, 7 d) and Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (50 ng/ml, 7 d). During the last 24 hours of treatment, Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/ml), Human Interleukin-1β (hIL-1β) #8900 (20 ng/ml), and Lipopolysaccharides (LPS) #14011 (1 μg/ml) were added to the cells. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using OX40L (D6X2D) Rabbit mAb (upper) and β-Actin (D6A8) #8457 (lower).

Background

OX40 (TNFRSF4, CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily that regulates T cell activity and immune responses. The OX40 protein contains four cysteine rich domains, a transmembrane domain, and a cytoplasmic tail containing a QEE motif (1,2). OX40 is primarily expressed on activated CD4+ and CD8+ T-cells, while the OX40 ligand (OX40L, TNFSF4, CD252) is predominantly expressed on activated antigen presenting cells (3-7). The engagement of OX40 with OX40L leads to the recruitment of TNF receptor-associated factors (TRAFs) and results in the formation of a TCR-independent signaling complex. One component of this complex, PKCθ, activates the NF-κB pathway (2,8). OX40 signaling through Akt can also enhance TCR signaling directly (9). Research studies indicate that the OX40L-OX40 pathway is associated with inflammation and autoimmune diseases (10). Additional research studies show that OX40 agonists augment anti-tumor immunity in several cancer types (11,12).

  1. Croft, M. (2010) Annu Rev Immunol 28, 57-78.
  2. So, T. and Croft, M. (2012) Front Immunol 3, 133.
  3. Paterson, D.J. et al. (1987) Mol Immunol 24, 1281-90.
  4. Mallett, S. et al. (1990) EMBO J 9, 1063-8.
  5. Dürkop, H. et al. (1995) Br J Haematol 91, 927-31.
  6. Godfrey, W.R. et al. (1994) J Exp Med 180, 757-62.
  7. Al-Shamkhani, A. et al. (1997) J Biol Chem 272, 5275-82.
  8. So, T. et al. (2011) Proc Natl Acad Sci U S A 108, 2903-8.
  9. So, T. and Croft, M. (2013) Front Immunol 4, 139.
  10. Gough, M.J. and Weinberg, A.D. (2009) Adv Exp Med Biol 647, 94-107.
  11. Weinberg, A.D. et al. (2011) Immunol Rev 244, 218-31.
  12. Linch, S.N. et al. (2015) Front Oncol 5, 34.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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