Cell Signaling Technology

Product Pathways - Autophagy Signaling

IRGM Antibody (Rodent Specific) #14979

No. Size Price
14979S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14979 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse,Rat, Endogenous 40 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

IRGM Antibody (Rodent Specific) recognizes endogenous levels of total IRGM protein in mouse and rat.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg215 of mouse IRGM protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse IRGM protein (mIRGM-Myc/DDK; +), using IRGM Antibody (Rodent Specific) (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from Raw 264.7 and J774A.1 cells, untreated (-) or treated with LPS #14011 (1 μg/ml, overnight; +), using IRGM Antibody (Rodent Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Immunity-related GTPase family M protein 1 (IRGM, LRG-47) belongs to the p47 family of immunity related guanosine triphosphatases (IRGs) that regulate innate immune responses to intracellular pathogens (1-3). Research studies indicate that IRGM plays a role in autophagy during clearance of intracellular bacteria (4). Expression of IRGM in mice, but not in humans, is induced by inflammatory signals that include interferon and LPS (2,3). Polymorphisms in the corresponding IRGM gene are associated with some cases of tuberculosis (5-7), Crohn’s disease (8,9), and severe sepsis (10). Additional studies indicate that IRGM functions through regulation of autophagy (4). Mitochondrial IRGM plays a role in mitochondrial fission, membrane polarization, and mitophagy (11). Knockout mice for IRGM show increased susceptibility to infection as well as intestinal inflammation and Paneth cell abnormalities (12,13). Knockout mice against IRGM are also resistant to neuronal autophagy following stroke (14). RNA viruses commonly target IRGM in order to suppress autophagy and enhance infection (15).

  1. Kim, B.H. et al. (2012) Cell Host Microbe 12, 432-44.
  2. Gilly, M. and Wall, R. (1992) J Immunol 148, 3275-81.
  3. Sorace, J.M. et al. (1995) J Leukoc Biol 58, 477-84.
  4. Singh, S.B. et al. (2006) Science 313, 1438-41.
  5. Che, N. et al. (2010) Clin Chim Acta 411, 1645-9.
  6. Intemann, C.D. et al. (2009) PLoS Pathog 5, e1000577.
  7. King, K.Y. et al. (2011) PLoS One 6, e16317.
  8. Parkes, M. et al. (2007) Nat Genet 39, 830-2.
  9. McCarroll, S.A. et al. (2008) Nat Genet 40, 1107-12.
  10. Kimura, T. et al. (2014) PLoS One 9, e91522.
  11. Singh, S.B. et al. (2010) Nat Cell Biol 12, 1154-65.
  12. Feng, C.G. et al. (2004) J Immunol 172, 1163-8.
  13. Liu, B. et al. (2013) Am J Physiol Gastrointest Liver Physiol 305, G573-84.
  14. He, S. et al. (2012) Autophagy 8, 1621-7.
  15. Grégoire, I.P. et al. (2011) PLoS Pathog 7, e1002422.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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