Product Pathways - Cell Cycle / Checkpoint
CENP-E Antibody #14977
|14977S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|14977||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting,
Specificity / Sensitivity
CENP-E Antibody recognizes endogenous levels of total CENP-E protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1897 of human CENP-E protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from U-2 OS cells treated with Nocodazole #2190 (10 nM, 16 hr) and released from mitosis into fresh medium for indicated times using CENP-E Antibody (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Centromere-associated protein E (CENP-E) is a kinesin-like motor protein and mitotic-checkpoint kinase BUB1B binding partner that is essential for establishing and maintaining stable attachments between mitotic chromosomes and spindle microtubules (1). CENP-E plays an important role as a motor protein in the alignment of chromosomes during prometaphase (2). Research studies indicate that CENP-E protein expression peaks in late G2 and M-phases of the cell cycle before the protein is degraded at mitotic exit (3). Additional studies show that the loss of CENP-E function results in cell cycle arrest in mitosis. Mutations in the corresponding CENPE gene can result in autosomal recessive primary microcephaly-13, a developmental disorder characterized by small head circumference, dysmorphic facial features, short stature, and delayed psychomotor development (4). Since CENP-E is essential for mitotic progression and is required for cellular proliferation, it has become an interesting target for cancer therapy (5-7).
- Yao, X. et al. (2000) Nat Cell Biol 2, 484-91.
- Yardimci, H. et al. (2008) Proc Natl Acad Sci U S A 105, 6016-21.
- Brown, K.D. et al. (1994) J Cell Biol 125, 1303-12.
- Mirzaa, G.M. et al. (2014) Hum Genet 133, 1023-39.
- Wood, K.W. et al. (2008) Clin Cancer Res 14, 7588-92.
- Balamuth, N.J. et al. (2010) Cancer Res 70, 2749-58.
- Kung, P.P. et al. (2014) Mol Cancer Ther 13, 2104-15.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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