Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

CDCA2 (D7T4P) Rabbit mAb #14976

No. Size Price
14976S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14976 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 140-150 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CDCA2 (D7T4P) Rabbit mAb recognizes endogenous levels of total CDCA2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr50 of human CDCA2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CDCA2 (D7T4P) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of ACHN cell extracts, untreated (-) or synchronized in mitosis by treatment with Nocodazole #2190 (10 nM, 16 hr; +), using CDCA2 (D7T4P) Rabbit mAb.

Background

Cell division cycle associated 2 (CDCA2, Repo-Man) is a cell-cycle protein that recruits protein phosphatase 1 (PP1) to mitotic chromatin at anaphase onset, which is essential for cell proliferation (1). Carboxy-terminal phosphorylation of CDCA2 at Ser893 by Aurora B inhibits the protein and leads to diffuse localization during prometaphase and metaphase. Dephosphorylation of CDCA2 by PP2A is necessary for CDCA2/PP1 complex reformation (2). The CDCA2/PP1 complex is required for chromatin binding and dephosphorylation of histone H3 at Thr3, Ser10, and Ser28 (2-4). The CDCA2/PP1 complex is also involved in nuclear envelope reformation during mitotic exit for proper progression through the M/G1 transition (4). The interaction of CDCA2 with importin beta and Nup153, which is required for nuclear envelope formation, is negatively regulated by CDK phosphorylation of the amino-terminal domain of CDCA2 (5). CDCA2 may play a role in DNA repair as the release of CDCA2 from chromatin at sites of DNA damage promotes the activation of DNA damage response (6). These results imply that the CDCA2/PP1 complex may play a part in cancer progression. Research studies indicate that CDCA2 may serve as a prognostic marker, as increased CDCA2 expression is seen in a number of cancers, including melanoma, neuroblastoma tumors, squamous cell carcinoma, and synovial sarcomas (7-9).

  1. Trinkle-Mulcahy, L. et al. (2006) J Cell Biol 172, 679-92.
  2. Qian, J. et al. (2013) Curr Biol 23, 1136-43.
  3. Adams, R.R. et al. (2001) J Cell Biol 153, 865-80.
  4. Vagnarelli, P. et al. (2011) Dev Cell 21, 328-42.
  5. Vagnarelli, P. and Earnshaw, W.C. (2012) Nucleus 3, 138-42.
  6. Peng, A. et al. (2010) Curr Biol 20, 387-96.
  7. Vagnarelli, P. (2014) Adv Exp Med Biol 773, 401-14.
  8. Uchida, F. et al. (2013) PLoS One 8, e56381.
  9. Lagarde, P. et al. (2013) J Clin Oncol 31, 608-15.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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