Cell Signaling Technology

Product Pathways - Neuroscience

β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb #14975

AD   alzheimer   amyloid   plaque  

No. Size Price
14975S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
14975T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
14975 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IHC-P 1:100 Human, Endogenous 4 Rabbit IgG
IF-F 1:200

Species cross-reactivity is determined by western blot.

Applications Key: IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb recognizes recombinant pE3 form of β-amyloid peptides. This antibody does not cross-react with the non-pyroglutamate (E3) form of β-amyloid peptides.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of β-amyloid (pE3) peptide.



Confocal immunofluorescent analysis of wild-type (left) and Tg2576 mouse model of Alzheimer's brain (right) using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Tg2576 mouse model of Alzheimer's brain using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of the indicated amounts of human Aβ (pE3-42) and Aβ (1-42) peptides using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb (upper) and β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb #12843 (lower).

Western Blotting

Western Blotting

β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb specificity is demonstrated using peptide dot blot. β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb binds to pre-coated β-amyloid (pE3) peptide but not to β-amyloid (E3) peptide or scrambled β-amyloid (pE3) peptide.


Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).

Aβ peptides can be further modified by amino-terminal truncation that exposes a free glutamate residue to the enzyme glutaminyl cyclase, which catalyzes the formation of an amino-terminal pyroglutamate (pE) (7,8). Aβ (pE3) peptides exhibit increased stability relative to non-modified peptides due to an enhanced resistance to peptidase-mediated degradation (9) and a higher propensity to form β-sheets and aggregate (10). Antibodies targeting Aβ (pE3) peptides may be plaque-specific as there is no evidence for circulating Aβ (pE3) peptides (11).

  1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
  2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.
  3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
  4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
  5. Ando, K. et al. (1999) J Neurosci 19, 4421-7.
  6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.
  7. Jawhar, S. et al. (2011) J Biol Chem 286, 38825-32.
  8. Saido, T.C. et al. (1995) Neuron 14, 457-66.
  9. Saido, T.C. et al. (1996) Neurosci Lett 215, 173-6.
  10. He, W. and Barrow, C.J. (1999) Biochemistry 38, 10871-7.
  11. Demattos, R.B. et al. (2012) Neuron 76, 908-20.

Application References

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