Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

TIM-1 (E1R9N) Rabbit mAb #14971

No. Size Price
14971S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14971 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 50, 90-140 Rabbit IgG
IHC-P 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

TIM-1 (E1R9N) Rabbit mAb recognizes endogenous levels of total TIM-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val24 of human TIM-1 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded normal human kidney using TIM-1 (E1R9N) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded ACHN (left) and HeLa (right) cell pellets using TIM-1 (E1R9N) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using TIM-1 (E1R9N) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using TIM-1 (E1R9N) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of ACHN cells (blue) and HeLa cells (red) using TIM-1 (E1R9N) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TIM-1 (E1R9N) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human TIM-1 (hTIM-1-DDK, +), using TIM-1 (E1R9N) Rabbit mAb.

Background

T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-1 (HAVCR1 (hepatitis A virus cellular receptor 1), KIM-1 (kidney injury molecule-1) was originally identified as a receptor for hepatitis A virus (1). TIM-1 also acts as a costimulatory receptor on T cells and following activation, associates with the TCR complex to upregulate signaling and cytokine production (2-5). Another TIM family member, TIM-4, is expressed by antigen presenting cells and is a ligand for TIM-1 (6). TIM-1 expressed by Th1 and Th17 cells was also recently shown to interact with P-selectin to mediate T cell trafficking during inflammation and autoimmune disease (7). NKT cells also express TIM-1, and engagement of TIM-1 on NKT cells leads to increased production of IL-4, but decreased production of IFN-gamma (8). TIM-1 is also a receptor for phosphatidylserine exposed by cells undergoing apoptosis. Detection of phosphatidylserine by TIM-1 expressed on NKT cells results in activation, proliferation, and cytokine production (9). Expression of TIM-1 on regulatory B cells is required for optimal production of IL-10. Mice lacking the TIM-1 mucin domain have decreased production of IL-10 by regulatory B cells, hyperactive T cells, increased levels of inflammatory cytokines, and enhanced severity of autoimmune disease (10,11). In addition, TIM-1 polymorphisms are associated with susceptibility to atopic diseases including asthma (12,13). Finally, expression of TIM-1 is increased in renal tubular epithelial cells following kidney injury (14).

  1. Kaplan, G. et al. (1996) EMBO J 15, 4282-96.
  2. Umetsu, S.E. et al. (2005) Nat Immunol 6, 447-54.
  3. de Souza, A.J. et al. (2005) Proc Natl Acad Sci U S A 102, 17113-8.
  4. Binné, L.L. et al. (2007) J Immunol 178, 4342-50.
  5. de Souza, A.J. et al. (2008) J Immunol 180, 6518-26.
  6. Meyers, J.H. et al. (2005) Nat Immunol 6, 455-64.
  7. Angiari, S. et al. (2014) Immunity 40, 542-53.
  8. Kim, H.S. et al. (2010) J Immunol 184, 4095-106.
  9. Lee, H.H. et al. (2010) J Immunol 185, 5225-35.
  10. Xiao, S. et al. (2012) Proc Natl Acad Sci U S A 109, 12105-10.
  11. Xiao, S. et al. (2015) J Immunol 194, 1602-8.
  12. McIntire, J.J. et al. (2001) Nat Immunol 2, 1109-16.
  13. Khademi, M. et al. (2004) J Immunol 172, 7169-76.
  14. Ichimura, T. et al. (1998) J Biol Chem 273, 4135-42.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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