Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

CXCL10 (D5L5L) Rabbit mAb #14969

No. Size Price
14969S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14969 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 10 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CXCL10 (D5L5L) Rabbit mAb recognizes endogenous levels of total CXCL10 protein. This antibody cross-reacts with a 100 kDa protein of unknown origin in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human CXCL10 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (50 ng/ml, 16 hr; +), using CXCL10 (D5L5L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of recombinant human CXCL10 protein using CXCL10 (D5L5L) Rabbit mAb.

Background

Chemokine (C-X-C motif) ligand 10 (CXCL10, IP-10) is a chemokine produced by a number of cell types and that is induced in response to pro-inflammatory cytokines, such as IFN-γ and TNF-α (1,2). CXCL10 binds to the chemokine receptor CXCR3 and induces calcium flux and cell migration (3). The CXCR3 protein is normally expressed on activated CD4+ T cells and NK cells, which can produce pro-inflammatory cytokines, including IFN-γ and TNF-α (3,4). As a result, these molecules act in an amplification loop to promote immune responses in the contexts of both autoimmunity and a tumor immune response (5,6).

  1. Shin, S.Y. et al. (2010) J Biol Chem 285, 30731-40.
  2. Luster, A.D. and Ravetch, J.V. (1987) J Exp Med 166, 1084-97.
  3. Loetscher, M. et al. (1996) J Exp Med 184, 963-9.
  4. Kajitani, K. et al. (2012) Breast Cancer Res Treat 134, 139-55.
  5. Luster, A.D. and Leder, P. (1993) J Exp Med 178, 1057-65.
  6. Antonelli, A. et al. (2014) Autoimmun Rev 13, 272-80.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications