Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

α-Smooth Muscle Actin Antibody #14968

sc-32251  

No. Size Price
14968S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14968 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 42 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

α-Smooth Muscle Actin Antibody recognizes endogenous levels of total α-smooth muscle actin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human α-smooth muscle actin protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various tissue using α-Smooth Muscle Actin Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, α-skeletal muscle extracts are negative for smooth muscle actin.

Background

Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility (1). The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells (1).

  1. Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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