Cell Signaling Technology

Product Pathways - Protein Stability

VPRBP (D5K5V) Rabbit mAb #14966

Cullin-RING Ubiquitin Ligases   DCAFs   Vpr (HIV-1) binding protein   Vpr-binding protein   VPRBP  

No. Size Price
14966S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14966 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 170 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Hamster, Pig,

Specificity / Sensitivity

VPRBP (D5K5V) Rabbit mAb recognizes endogenous levels of total VPRBP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe1184 of human VPRBP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using VPRBP (D5K5V) Rabbit mAb.

Background

The HIV-1 viral protein R (Vpr)-binding protein (VPRBP, DCAF1) is a substrate-specific adaptor for the CUL4-based ubiquitin ligase complex that consists of CUL4A, RBX1, and DDB1 (1). VPRBP protein structure contains a central LIS1 homology (LisH) motif responsible for dimerization, and two carboxy-terminal WD-40 motifs involved in Vpr and DDB1 binding (2-4). Research studies demonstrate that VPRBP plays a role in hepatic lipid metabolism by promoting the ubiquitin-dependent proteasomal degradation of the TR4 nuclear receptor, which is involved in lipid homeostasis (5). The VPRBP protein plays a role in mammalian germ cell development through regulation of TET methylcytosine dioxygenase activation (6). Additional studies show that VPRBP exhibits kinase activity and phosphorylates histone H2A at Ser120, which blocks tumor suppressor gene transcription (7). The tumor suppressor Merlin/NF2 inhibits tumorigenesis through interaction with and suppression of the CUL4A-RBX1-DDB1-VPRBP complex (8).

  1. Lee, J. and Zhou, P. (2007) Mol Cell 26, 775-80.
  2. Le Rouzic, E. et al. (2007) Cell Cycle 6, 182-8.
  3. Zhang, S. et al. (2001) Gene 263, 131-40.
  4. Le Rouzic, E. et al. (2008) J Biol Chem 283, 21686-92.
  5. Yoshizawa, T. et al. (2014) Cell Metab 19, 712-21.
  6. Yu, C. et al. (2013) Science 342, 1518-21.
  7. Kim, K. et al. (2013) Mol Cell 52, 459-67.
  8. Li, W. et al. (2010) Cell 140, 477-90.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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