Cell Signaling Technology

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Phospho-CDCP1 (Tyr743) (D2G2J) Rabbit mAb #14965

No. Size Price
14965S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
14965 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 140, 70 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-CDCP1 (Tyr743) (D2G2J) Rabbit mAb recognizes endogenous levels of CDCP1 protein only when phosphorylated at Tyr743. This antibody may cross-react with other tyrosine-phosphorylated proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr743 of human CDCP1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from DLD-1 cells, untreated (-) or treated with a Cub1 antibody (+), using Phospho-CDCP1 (Tyr743) (D2G2J) Rabbit mAb (upper) and CDCP1 (D1W9N) Rabbit mAb #13794 (lower).

Background

CUB domain containing protein 1 (CDCP1, SIMA135) is a putative stem cell marker shown in research studies to be highly expressed in some human cancer cells and in both typical and atypical (cancerous) colons (1). Expression of CDCP1 may be epigenetically regulated, as methylation of promoter CpG sequences results in decreased CDCP1 expression (2). The corresponding CDCP1 gene encodes a glycoprotein that acts as a complex, multidomain transmembrane antigen. CDCP1 has three extracellular CUB domains that may be involved in cell adhesion or extracellular matrix interactions (1,3). Src-family kinases may phosphorylate CDCP1 at five tyrosine residues within its cytoplasmic domain to provide a potential binding site for SH2 domain-containing proteins (3). CDCP1 is a putative hematopoietic stem cell marker (4,5).

  1. Scherl-Mostageer, M. et al. (2001) Oncogene 20, 4402-8.
  2. Ikeda, J.I. et al. (2006) J. Pathol. 210, 75-84.
  3. Hooper, J.D. et al. (2003) Oncogene 22, 1783-94.
  4. Conze, T. et al. (2003) Ann. N.Y. Acad. Sci. 996, 222-226.
  5. Bühring, H.J. et al. (2004) Stem Cells 22, 334-343.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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