Cell Signaling Technology

Product Pathways - Angiogenesis

PROX1 (D2J6J) Rabbit mAb #14963

No. Size Price
14963S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14963 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 85 Rabbit IgG
IHC-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

PROX1 (D2J6J) Rabbit mAb recognizes endogenous levels of total PROX1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu567 of human PROX1 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using PROX1 (D2J6J) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PROX1 (D2J6J) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PROX1 (D2J6J) Rabbit mAb in the presence of the control peptide (left) or antigen-specific peptide (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PROX1 (D2J6J) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, MDA-MB-231 cells are negative for PROX1 expression.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using PROX1 (D2J6J) Rabbit mAb.

Background

Prospero homeobox protein 1 (PROX1) is a transcription factor known for its roles in organ development and lymphangiogenesis. It plays a critical role in the development of the CNS, lens, retina, liver, pancreas, and heart, and is considered to be the master regulator of the lymphatic system (1,2). PROX1 initiates the differentiation of lymphatic vasculature from the cardinal vein, where it is regulated by Sox18 (3,4). The PROX1 suppressor COUP-TFII represses the Notch pathway in venous endothelium, which prevents arterialization (4). HIF-1α and HIF-1β mediated hypoxia induces PROX1, which suggests a means of promoting lymphangiogenesis. Since the tumor microenvironment is typically hypoxic, regulation of PROX1 by hypoxia may also explain the up-regulation of this transcription factor in some cancers (2). PROX1 promotes colon cancer progression by down-regulating E-cadherin via miR-9, which promotes epithelial-mesenchymal transition (EMT) and metastasis (5). The PROX1 protein can act as a tumor suppressor in cases of hepatocellular carcinoma. PROX1 represses transcription of TWIST1, a transcription factor that promotes metastasis by binding the E-cadherin promoter. The function of PROX1 in other cancers is an area of active research (6).

  1. Shan, S.F. et al. (2008) FEBS Lett 582, 3723-8.
  2. Zhou, B. et al. (2013) FEBS Lett 587, 724-31.
  3. Aranguren, X.L. et al. (2013) J Cell Sci 126, 1164-75.
  4. Kim, H. et al. (2013) PLoS One 8, e52197.
  5. Lu, M.H. et al. (2012) Clin Cancer Res 18, 6416-25.
  6. Chang, T.M. and Hung, W.C. (2012) FEBS Lett 586, 3746-52.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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