Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

Sox2 (D1C7J) XP® Rabbit mAb #14962

sc-17320   sc-20088  

No. Size Price
14962S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
14962T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
14962 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 35 Rabbit IgG
IHC-P 1:300

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Homology

Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Sox2 (D1C7J) XP® Rabbit mAb recognizes endogenous levels of total Sox2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly152 of human Sox2 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse forestomach using Sox2 (D1C7J) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded E14.5 embryonic mouse lung using Sox2 (D1C7J) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NTERA-2 cl.D1 (left) and HeLa (right) cell pellets using Sox2 (D1C7J) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Sox2 (D1C7J) XP® Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from F9 and NTERA-2 cl.D1 cells using Sox2 (D1C7J) XP® Rabbit mAb.

Background

Embryonic stem cells (ESC) derived from the inner cell mass of the blastocyst are unique in their pluripotent capacity and potential for self-renewal (1). Research studies demonstrate that a set of transcription factors that includes Oct-4, Sox2, and Nanog forms a transcriptional network that maintains cells in a pluripotent state (2,3). Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development (4,5). siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (6). Induced overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram both mouse and human somatic cells to a pluripotent state (7,8). Additional evidence demonstrates that Sox2 is also present in adult multipotent progenitors that give rise to some adult epithelial tissues, including several glands, the glandular stomach, testes, and cervix. Sox2 is thought to regulate target gene expression important for survival and regeneration of these tissues (9).

  1. Conley, B.J. et al. (2004) Int J Biochem Cell Biol 36, 555-67.
  2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.
  3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
  4. Boyer, L.A. et al. (2005) Cell 122, 947-56.
  5. Loh, Y.H. et al. (2006) Nat Genet 38, 431-40.
  6. Matin, M.M. et al. (2004) Stem Cells 22, 659-68.
  7. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
  8. Okita, K. et al. (2007) Nature 448, 313-7.
  9. Arnold, K. et al. (2011) Cell Stem Cell 9, 317-29.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

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For Research Use Only. Not For Use In Diagnostic Procedures.

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XP is a registered trademark of Cell Signaling Technology, Inc.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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