Cell Signaling Technology

Product Pathways - Transcription Factors

IRX1 (D5S8Y) Rabbit mAb #14961

Iroquois  

No. Size Price
14961S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14961 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 50 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

IRX1 (D5S8Y) Rabbit mAb recognizes endogenous levels of total IRX1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro466 of human IRX1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IRX1 (D5S8Y) Rabbit mAb.

IP

IP

Immunoprecipitation of IRX1 from WERI-Rb-1 cell lysates using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IRX1 (D5S8Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IRX1 (D5S8Y) Rabbit mAb.

Background

The Iroquois-class homeodomain proteins (IRX1-6) comprise a family of transcription factors, initially identified in a mutagenesis experiment in Drosophila designed to identify genes involved in embryonic patterning (1, 2). Members of the Iroquois-class protein family contain a three amino acid loop extension (TALE) homeodomain and an IRO sequence motif of unknown function that is unique to this protein family, but which bears similarity to Notch receptor domains involved in protein-protein interactions (3, 4). Research studies in various animal models suggest important roles for IRX proteins in early development, including neural patterning, heart field specification, kidney development and lung development (5-8). Moreover, studies in mice have shown an essential role for IRX proteins in eye development, where they are required for the specification of ommatidia and photoreceptor cells (9).

The human IRX1 gene has been identified as a candidate tumor suppressor (10) that is hypermethylated in a number of cancer subtypes (11). Research studies in head and neck squamous cell carcinoma cell lines specifically suggest that IRX1 interacts with components of the TGFβ signaling pathway to regulate cell proliferation and differentiation (12).

  1. Dambly-Chaudière, C. and Leyns, L. (1992) Int J Dev Biol 36, 85-91.
  2. Leyns, L. et al. (1996) Mech Dev 59, 63-72.
  3. Cavodeassi, F. et al. (2001) Development 128, 2847-55.
  4. Lai, E.C. et al. (1998) Development 125, 4077-88.
  5. Bosse, A. et al. (1997) Mech Dev 69, 169-81.
  6. Christoffels, V.M. et al. (2000) Dev Biol 224, 263-74.
  7. Marra, A.N. and Wingert, R.A. (2014) Cell Dev Biol 3, 1000131.
  8. Becker, M.B. et al. (2001) Mech Dev 106, 155-8.
  9. Choy, S.W. et al. (2010) Dev Dyn 239, 3204-14.
  10. Mahoney, S.E. et al. (2012) Epigenetics 7, 400-8.
  11. Bennett, K.L. et al. (2008) Cancer Res 68, 4494-9.
  12. Bennett, K.L. et al. (2009) Cancer Res 69, 9301-5.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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