Cell Signaling Technology

Product Pathways - Neuroscience

CGRP (D5R8F) Rabbit mAb #14959

neuropeptide   sc-28920  

No. Size Price
14959S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14959T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
14959 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 5 Rabbit IgG
IF-F 1:400
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

CGRP (D5R8F) Rabbit mAb recognizes endogenous levels of total CGRP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val114 of human CGRP protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of TT (left, positive) and HeLa (right, negative) cells using CGRP (D5R8F) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-F

IF-F

Confocal immunofluorescent analysis of C1 rat spinal cord, 10X magnification (left) and 20X magnification (right), using CGRP (D5R8F) Rabbit mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from TT and HeLa cells using CGRP (D5R8F) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of the indicated amounts of human CGRP using CGRP (D5R8F) Rabbit mAb.

Background

Calcitonin gene-related peptide (CGRP) is a peptide of 37 amino acids that belongs to the calcitonin (CT) family of peptide hormones. The calcitonin gene (CALCA) encodes a number of tissue-specific peptides through alternative splicing of mRNA transcripts and precursor protein cleavage. Both calcitonin and α-CGRP are produced from the CALCA gene, while a second gene (CALCB) encodes the related β-CGRP protein (1). α-CGRP and β-CGRP share similar activities and differ by three or fewer residues depending on the species (3). The CGRP peptide activates a heterotrimeric receptor complex that consists of the seven transmembrane-spanning calcitonin receptor-like receptor, the single transmembrane-spanning RAMP1 protein, and an intracellular receptor component protein (4,5). CGRP is expressed in the central and peripheral nervous system in mammals, where it exhibits several important physiologic roles. Research studies demonstrate that CGRP is a potent vasodilatator (6) and a modulator of acetylcholine receptor function at neuromuscular junctions (7). Additional studies indicate that CGRP peptide is involved in feeding (8) and inflammatory pain (9). CGRP peptide also plays a key role in the physiology of migraine attacks. Specifically, CGRP peptide levels increase during acute migraine attacks, which can be ameliorated through treatment with CGRP antagonists (10).

  1. Rosenfeld, M.G. et al. (1983) Nature 304, 129-35.
  2. Amara, S.G. et al. (1985) Science 229, 1094-7.
  3. Breimer, L.H. et al. (1988) Biochem J 255, 377-90.
  4. McLatchie, L.M. et al. (1998) Nature 393, 333-9.
  5. Evans, B.N. et al. (2000) J Biol Chem 275, 31438-43.
  6. Struthers, A.D. et al. (1986) Clin Sci (Lond) 70, 389-93.
  7. New, H.V. and Mudge, A.W. (1986) Nature 323, 809-11.
  8. Lutz, T.A. et al. (1997) Neurosci Lett 230, 159-62.
  9. Birklein, F. and Schmelz, M. (2008) Neurosci Lett 437, 199-202.
  10. Ho, T.W. et al. (2010) Nat Rev Neurol 6, 573-82.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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