Cell Signaling Technology

Product Pathways - DNA Damage

p95/NBS1 (D6J5I) Rabbit mAb #14956

No. Size Price
14956S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
14956 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 95 Rabbit IgG
IP 1:200
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

p95/NBS1 (D6J5I) Rabbit mAb recognizes endogenous levels of total p95/NBS1 protein. This antibody also cross-reacts with an unidentified protein of 180 kDa in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala740 of human p95/NBS1 protein.



Confocal immunofluorescent analysis of SK-MEL-28 (left) and GM07166 (right) cells using p95/NBS1 (D6J5I) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using p95/NBS1 (D6J5I) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).



Immunoprecipitation of p95/NBS1 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or p95/NBS1 (D6J5I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using p95/NBS1 (D6J5I) Rabbit mAb.


Nijmegen breakage syndrome (NBS) is characterized by growth retardation, mental disability, immunodeficiency, defects in cell cycle checkpoints, an increased propensity for cancer, and sensitivity to ionizing radiation (1). Repair of radiation-induced DNA double-strand breaks is dependent on the multifunctional MRN complex containing Mre11, Rad50, and the NBS1 gene product p95/NBS1 (also called p95 or nibrin) (2). p95/NBS1 is a protein with a forkhead-associated domain and a BRCT repeat that regulate interaction with MDC1 and are essential for proper G2/M DNA-damage checkpoint function (3). NBS1 is critical for homologous recombination following DNA double strand breaks. This activity requires CDK-dependent association with CtIP and subsequent phosphorylation by ATM (4). ATM interacts with and phosphorylates p95/NBS1 at Ser278 and Ser343 after exposure to ionizing radiation (5,6).

  1. Chrzanowska, K.H. et al. (2012) Orphanet J Rare Dis 7, 13.
  2. Lee, J.H. et al. (2013) J Biol Chem 288, 12840-51.
  3. Hari, F.J. et al. (2010) EMBO Rep 11, 387-92.
  4. Wang, H. et al. (2013) PLoS Genet 9, e1003277.
  5. Zhao, S. et al. (2000) Nature 405, 473-7.
  6. Wen, J. et al. (2013) Oncogene 32, 4448-56.

Application References

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