Product Pathways - MAPK Signaling
Spry2 (D3G1A) Rabbit mAb #14954
|14954S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|14954||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
Spry2 (D3G1A) Rabbit mAb recognizes endogenous levels of total Spry2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro71 of human Spry2 protein.
Western blot analysis of extracts from various cell lines using Spry2 (D3G1A) Rabbit mAb.
The Sprouty (Spry) family of proteins are antagonists of receptor tyrosine kinase (RTK)-induced signaling (1, 2). The Spry proteins play crucial roles in regulating growth and development of living organisms. Since originally discovered in Drosophila, four human orthologs of Spry proteins (Spry1-4) have been identified. All human Spry proteins possess a conserved carboxyl-terminal cysteine-rich SPR domain, which harbors a signal for protein translocation from cytosol to membrane ruffles (3,4). The SPR domain also enables the Spry proteins to form homo- or hetero-dimers and to interact with other proteins including kinases and phosphatases. The SPR domain is essential for the inhibitory modulation of Spry proteins on RTK signaling (1,2).
Studies have shown that several cancers have reduced levels of Spry2 expression implicating Spry2 as a tumor suppressor (5-8). The regulation of Spry2 expression and activity appears to be a complex process involving casein kinase 1, Shp2 phosphatase, and Spry2-interacting partners (9-11). Phosphorylation of Tyr55 residue of Spry2 is required for the inhibitory function of Spry2 in FGF/MAPK signaling (12,13).
- Guy, G.R. et al. (2003) J Cell Sci 116, 3061-8.
- Guy, G.R. et al. (2009) J Endocrinol 203, 191-202.
- Lim, J. et al. (2000) J Biol Chem 275, 32837-45.
- Lim, J. et al. (2002) Mol Cell Biol 22, 7953-66.
- Herold, T. et al. (2014) Blood 124, 1304-11.
- Gao, M. et al. (2012) EMBO Mol Med 4, 776-90.
- Sánchez, A. et al. (2008) Oncogene 27, 4969-72.
- Frank, M.J. et al. (2009) Blood 113, 2478-87.
- Yim, D.G. et al. (2015) Oncogene 34, 474-84.
- Okur, M.N. et al. (2014) Mol Cell Biol 34, 271-9.
- Mason, J.M. et al. (2004) Mol Biol Cell 15, 2176-88.
- Edwin, F. et al. (2009) Mol Pharmacol 76, 679-91.
- Fong, C.W. et al. (2003) J Biol Chem 278, 33456-64.
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