Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-NuMA (Thr2055) Antibody #14951

No. Size Price
14951S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
14951 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 238 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-NuMA (Thr2055) Antibody recognizes endogenous levels of NuMA protein only when phosphorylated at Thr2055.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr2055 of human NuMA protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of HT-29 cell extracts, untreated (-) or treated with thymidine (2 mM, 16 hr) followed by Nocodazole #2190 (10 nM, 24 hr; +), using Phospho-NuMA (Thr2055) Antibody (upper), NuMA (D49H4) Rabbit mAb #8967 (middle), and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).


The nuclear mitotic apparatus protein (NuMA) is a coiled coil protein involved in the formation and maintenance of the mitotic spindle. NuMA plays a role in chromatin organization during interphase, which influences mammary epithelial differentiation (1,2). During apoptosis, carboxy-terminal cleavage of NuMA may amplify signaling in the cell death pathway (2). NuMA is phosphorylated at numerous sites, with phosphorylation at Ser395 occurring in an ATM/ATR-dependent manner in response to DNA damage (3,4).

Phosphorylation at Thr2055 by CDK1 is required for spindle pole association of NuMA at the onset of mitosis. Dephosphorylation by PPP2CA leads to enhancement of NuMA at the cell cortex in anaphase and proper cell-cycle progression (5,6).

  1. Abad, P.C. et al. (2007) Mol Biol Cell 18, 348-61.
  2. Lin, H.H. et al. (2007) J Biomed Sci 14, 681-94.
  3. Stokes, M.P. et al. (2007) Proc Natl Acad Sci USA 104, 19855-60.
  4. Matsuoka, S. et al. (2007) Science 316, 1160-6.
  5. Kotak, S. et al. (2013) EMBO J 32, 2517-29.
  6. Seldin, L. et al. (2013) Mol Biol Cell 24, 3651-62.

Application References

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