Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

MED26 (D4B1X) Rabbit mAb #14950

No. Size Price
14950S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14950 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 70 Rabbit IgG
IP 1:100
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

MED26 (D4B1X) Rabbit mAb recognizes endogenous levels of total MED26 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro120 of human MED26 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Raji, HeLa, and Daudi cells using MED26 (D4B1X) Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells heat shocked for 1 hr and either 5 µl of MED26 (D4B1X) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Myc Intron 1 Primers #14905, SimpleChIP® Human HSP70 Intron 1 Primers #14023, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

The mediator complex consists of about 25-30 proteins and is thought to facilitate transcription activation by acting as a molecular bridge between the RNA polymerase II (RNAPII) machinery and transcription factors (1). Mediator is recruited to target genes by transcription factors and plays an essential role in the recruitment and stabilization of the RNAPII transcription complex at promoters, as well as the activation of transcription post RNAPII recruitment (1-5). The mediator complex also plays an important role in creating ‘chromatin loops’ that occur as a result of interactions between the transcription factor bound at distal enhancers and RNAPII bound at the proximal promoter, and works to sustain proper chromatin architecture during active transcription (6-8).

Mediator complex subunit 26 (MED26) plays a critical role in the transition of RNAPII from the initiation to elongation phase of transcription. The amino-terminal domain of MED26 is required for recruitment of the ELL- and pTEFb-containing complexes to target genes, which facilitates transcription elongation (9).

  1. Taatjes, D.J. (2010) Trends Biochem Sci 35, 315-22.
  2. Kornberg, R.D. (2005) Trends Biochem Sci 30, 235-9.
  3. Malik, S. and Roeder, R.G. (2005) Trends Biochem Sci 30, 256-63.
  4. Malik, S. et al. (2002) Mol Cell Biol 22, 5626-37.
  5. Wang, G. et al. (2005) Mol Cell 17, 683-94.
  6. Kagey, M.H. et al. (2010) Nature 467, 430-5.
  7. Park, S.W. et al. (2005) Mol Cell 19, 643-53.
  8. Chen, Z. et al. (2011) EMBO J 30, 2405-19.
  9. Takahashi, H. et al. (2011) Cell 146, 92-104.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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