Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

TBC1D7 (D8K1Y) Rabbit mAb #14949

No. Size Price
14949S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14949 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 30 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

TBC1D7 (D8K1Y) Rabbit mAb recognizes endogenous levels of total TBC1D7 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TBC1D7 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, HeLa, and C2C12 cells using TBC1D7 (D8K1Y) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells expressing either GFP shRNA (shRNA-GFP) or TBC1D7 shRNA (shRNA-TBC1D7) using TBC1D7 (D8K1Y) Rabbit mAb and β-Actin (13E5) Rabbit mAb #4970.

Background

TBC1 domain family member 7 (TBC1D7, TBC7) belongs to a family of TBC (Tre-2/Bub2/Cdc16) containing proteins that function as GTPase-activating proteins (GAPs) (1,2). TBC1D7 was initially identified as a novel binding protein within the TSC1-TSC2 complex, where it was thought to associate with TSC1 (3,4). Additional research indicates that TBC1D7 is a third subunit of the TSC1-TSC2 complex that possesses Rheb-GAP activity and signals upstream of mTORC1 (5). Knockdown of TBC1D7 limits the association between TSC1 and TSC2, resulting in reduced Rheb-GAP activity and increased mTORC1 signaling (5). Mutations in the corresponding TBC1D7 gene result in increased mTORC1 signaling, delayed autophagy, and are associated with intellectual disability (ID) and macrocrania (6,7).

  1. Pan, X. et al. (2006) Nature 442, 303-6.
  2. Fukuda, M. (2011) Biosci Rep 31, 159-68.
  3. Nakashima, A. et al. (2007) Biochem Biophys Res Commun 361, 218-23.
  4. Sato, N. et al. (2010) Genes Chromosomes Cancer 49, 353-67.
  5. Dibble, C.C. et al. (2012) Mol Cell 47, 535-46.
  6. Capo-Chichi, J.M. et al. (2013) J Med Genet 50, 740-4.
  7. Alfaiz, A.A. et al. (2014) Hum Mutat 35, 447-51.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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