Cell Signaling Technology

Product Pathways - Screening Technologies

Malonyl-Lysine [Mal-K] MultiMab™ Rabbit mAb mix #14942

lysine malonylate   malonyl   malonyl-lysine   malonylation  

No. Size Price
14942S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
14942 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Malonyl-Lysine [Mal-K] MultiMab™ Rabbit mAb mix recognizes endogenous levels of proteins only when malonylated at a lysine residue. This antibody does not cross-react with other lysine modifications.

Source / Purification

MultiMab™ Rabbit mAb mixes are prepared by combining individual rabbit monoclonal clones in optimized ratios for the approved applications. Each antibody in the mix is carefully selected based on motif recognition and performance in multiple assays. Each mix is engineered to yield the broadest possible coverage of the modification being studied while ensuring a high degree of specificity for the modification or motif.

Western Blotting

Western Blotting

Western blot analysis of extracts from SIRT5 wild type (WT) and knockout (KO) mouse livers using Malonyl-Lysine [Mal-K] MultiMab™ Rabbit mAb mix (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Background

Lysine is subject to a wide array of regulatory post-translational modifications due to its positively charged ε-amino group side chain. The most prevalent of these are ubiquitination and acetylation, which are highly conserved among prokaryotes and eukaryotes (1,2). Acyl group transfer from the metabolic intermediates acetyl-, succinyl-, malonyl-, glutaryl-, butyryl-, propionyl-, and crotonyl-CoA all neutralize lysine’s positive charge and confer structural alterations affecting substrate protein function. Lysine acetylation is catalyzed by histone acetyltransferases, HATs, using acetyl-CoA as a cofactor (3,4). Deacylation is mediated by histone deacetylases, HDACs 1-11, and NAD-dependent Sirtuins 1-7. Some sirtuins have little to no deacetylase activity, suggesting that they are better suited for other acyl lysine substrates (5).

SIRT5 is a predominantly mitochondrial desuccinylase and demalonylase (5,6). In the absence of a known malonyltransferase, nonenzymatic protein malonylation is likely driven by the concentration of malonyl-CoA and intracellular pH and is subject to metabolic fluctuations (7). Malonylation is especially prevalent among mitochondrial metabolic proteins. In type II diabetes mouse models, notably elevated malonylation can be detected mainly, but not exclusively, on proteins of glucose and fatty acid metabolism (8). Yeast histone H3K56 malonylation suggests poor DNA binding efficiency and may lead to reduced cell viability (9).

  1. Liu, Z. et al. (2014) Nucleic Acids Res 42, D531-6.
  2. Lee, S. (2013) Toxicol Res 29, 81-6.
  3. Lin, H. et al. (2012) ACS Chem Biol 7, 947-60.
  4. Zhang, Z. et al. (2011) Nat Chem Biol 7, 58-63.
  5. Du, J. et al. (2011) Science 334, 806-9.
  6. Peng, C. et al. (2011) Mol Cell Proteomics 10, M111.012658.
  7. Newman, J.C. et al. (2012) J Biol Chem 287, 42436-43.
  8. Du, Y. et al. (2015) Mol Cell Proteomics 14, 227-36.
  9. Xie, Z. et al. (2012) Mol Cell Proteomics 11, 100-7.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

MultiMab is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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