Cell Signaling Technology

Product Pathways - Metabolism

Thioredoxin 2 (D1C9L) Rabbit mAb #14907

mitochondrial thioredoxin   Mt-Trx   MTRX   THIOM   thioredoxin 2   Thioredoxin-2   Thioredoxin. mitochondrial   TRX2   TXN2  

No. Size Price
14907S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14907 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey, Endogenous 13 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Thioredoxin 2 (D1C9L) Rabbit mAb recognizes endogenous levels of total thioredoxin 2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu100 of human thioredoxin 2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Thioredoxin 2 (D1C9L) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human thioredoxin 2 protein (hTRX2-Myc; +), using Thioredoxin 2 (D1C9L) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond that is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (TRX1) and mitochondrial thioredoxin 2 (TRX2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress, and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging, and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, because it acts as a negative regulator of the proapoptotic kinase ASK1 (3). Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5).

  1. Watson, W.H. et al. (2004) Toxicol Sci 78, 3-14.
  2. Burke-Gaffney, A. et al. (2005) Trends Pharmacol Sci 26, 398-404.
  3. Saitoh, M. et al. (1998) EMBO J 17, 2596-606.
  4. Callister, M.E. et al. (2007) Intensive Care Med 33, 364-7.
  5. Callister, M.E. et al. (2006) Thorax 61, 521-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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