Cell Signaling Technology

Product Pathways - Metabolism

Lipin 1 (D2W9G) Rabbit mAb #14906

DKFZp781P1796   KIAA0188   lipin 1   Lipin-1   LPIN1   PAP1   Phosphatidate phosphatase LPIN1  

No. Size Price
14906S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14906 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 130 Rabbit IgG
IP 1:100
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Lipin 1 (D2W9G) Rabbit mAb recognizes endogenous levels of total lipin 1 protein. This antibody may also cross-react with an unidentified protein of 35 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human lipin 1 protein.



Confocal immunofluorescent analysis of 3T3-L1 adipocytes (differentiated, 7 days) using Lipin 1 (D2W9G) Rabbit mAb (red). Lipid droplets were labeled with BODIPY® 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Lipin 1 (D2W9G) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).


Lipin 1 was identified as a nuclear protein required for adipose tissue development (1). The expression of Lipin 1 is induced during adipocyte differentiation (1). The abnormal development of adipose tissues caused by mutations in the lipin 1 gene results in lipodystrophy, a condition associated with low body fat, fatty liver, hypertriglyceridemia, and insulin resistance (1). Lipin 1 plays a role in lipid metabolism in various tissues and cell types including liver, muscle, adipose tissues, and neuronal cell lines (2-4). It has dual functions at the molecular level: Lipin 1 serves as a transcriptional coactivator in liver, and a phosphatidate phosphatase in triglyceride and phospholipid biosynthesis pathways (5). Lipin 1 is regulated by mTOR, illustrating a connection between adipocyte development and nutrient-sensing pathways (6). It also mediates hepatic insulin signaling by TORC2/CRTC2 (7).

  1. Péterfy, M. et al. (2001) Nat Genet 27, 121-4.
  2. Finck, B.N. et al. (2006) Cell Metab 4, 199-210.
  3. Phan, J. and Reue, K. (2005) Cell Metab 1, 73-83.
  4. Verheijen, M.H. et al. (2003) Genes Dev 17, 2450-64.
  5. Reue, K. and Zhang, P. (2008) FEBS Lett 582, 90-6.
  6. Huffman, T.A. et al. (2002) Proc Natl Acad Sci U S A 99, 1047-52.
  7. Ryu, D. et al. (2009) Cell Metab 9, 240-51.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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BODIPY is a registered trademark of Life Technologies Corporation.

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