Cell Signaling Technology

Product Pathways - Tag Antibodies

HA-Tag (C29F4) Rabbit mAb (PE Conjugate) #14904

sc-7392   sc-805  

No. Size Price
14904S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
14904 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 All Species Expected, Transfected Only Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

HA-Tag (C29F4) Rabbit mAb (PE Conjugate) detects exogenously expressed proteins containing the HA epitope tag.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA).

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated HA-Tag (C29F4) Rabbit mAb #3724.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of 293T cells, untransfected (blue) or transfected with HA-Tagged Akt (green), using HA-Tag (C29F4) Rabbit mAb (PE Conjugate).

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The HA tag is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors (1).

  1. Field, J. et al. (1988) Mol Cell Biol 8, 2159-65.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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