Cell Signaling Technology

Product Pathways - Transcription Factors

MIST1/bHLHa15 (D7N4B) XP® Rabbit mAb #14896

No. Size Price
14896S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
14896T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
14896 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 23 Rabbit IgG
IHC-P 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Homology

Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

MIST1/bHLHa15 (D7N4B) XP® Rabbit mAb recognizes endogenous levels of total MIST1/bHLHa15 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln181 of human MIST1/bHLHa15 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from human pancreas, mouse pancreas, and human colon using MIST1/bHLHa15 (D7N4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human pancreas using MIST1/bHLHa15 (D7N4B) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse pancreas using MIST1/bHLHa15 (D7N4B) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse spleen using MIST1/bHLHa15 (D7N4B) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse small intestine using MIST1/bHLHa15 (D7N4B) XP® Rabbit mAb.

Background

Class A basic helix-loop-helix protein 15 (MIST1, bHLHa15) is a highly conserved basic helix loop helix family transcription factor that binds E-box motifs and regulates the expression of developmentally regulated genes (1). MIST1 can bind DNA as a homodimer, or may heterodimerize with other bHLH proteins to regulate target gene expression (1). MIST1 is expressed in an array of tissues, including salivary glands, stomach, small intestine, and the pancreas, but is generally restricted to secretory cell subtypes (2). In the pancreas, MIST1 is essential for the maturation, maintenance, and function of acinar cells (3). In gastric chief cells, MIST1 regulates the expression of RAB26 and RAB3D, two GTPases that function to regulate secretory granule formation (4). Loss of MIST1 in gastric chief cells may be a potential marker of gastric epithelial neoplasia (5).

  1. Lemercier, C. et al. (1997) Dev Biol 182, 101-13.
  2. Pin, C.L. et al. (2000) Anat Rec 259, 157-67.
  3. Pin, C.L. et al. (2001) J Cell Biol 155, 519-30.
  4. Tian, X. et al. (2010) Mol Cell Biol 30, 1269-84.
  5. Lennerz, J.K. et al. (2010) Am J Pathol 177, 1514-33.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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