Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

MLLT1/ENL (D9M4B) Rabbit mAb #14893

No. Size Price
14893S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14893 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80 Rabbit IgG
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, ChIP=Chromatin IP,

Specificity / Sensitivity

MLLT1/ENL (D9M4B) Rabbit mAb recognizes endogenous levels of total MLLT1/ENL protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala343 of human MLLT1/ENL protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MLLT1/ENL (D9M4B) Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MV-4-11 cells and either 10 µl of MLLT1/ENL (D9M4B) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA9 Promoter Primers #14909, human MEIS1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

The super elongation complex (SEC) plays a critical role in regulating RNA polymerase II (RNAPII) transcription elongation (1). The SEC is composed of AFF4, AFF1/AF4, MLLT3/AF9, and MLLT1/ENL proteins. The pathogenesis of mixed lineage leukemia is often associated with translocations of the SEC subunits joined to the histone H3 Lys4 methyltransferase mixed lineage leukemia (MLL) gene (1-4). The SEC has been found to contain RNAPII elongation factors eleven-nineteen lysine-rich leukemia (ELL), ELL2, and ELL3, along with the associated factors EAF1 and EAF2, which can increase the catalytic rate of RNAPII transcription in vitro, (1,2,5-7). The SEC positive transcription elongation factor b (P-TEFb) phosphorylates the carboxy-terminal domain within the largest subunit of RNAP II at Ser2 of the heptapeptide repeat. The SEC negative transcription elongation factors, DRB-induced stimulating factor (DSIF) and negative elongation factor (NELF), signal the transition from transcription initiation and pausing to productive transcription elongation (2,8-10). The chromosomal translocation of MLL with the members of the SEC leads to SEC recruitment to MLL regulated genes, such as the highly developmentally regulated Hox genes, implicating the misregulation and overexpression of these genes as underlying contributors to leukemogenesis (1,2,9,11).

MLL translocated to 1/eleven-nineteen-leukemia (MLLT1/ENL) is also found as part of the histone H3 Lys79 methyltransferase disruptor of telomeric silencing-like (Dot1L) complex that has been suggested to play a role in transcription elongation. This complex regulates the expression of genes, such as the Wnt-signaling pathway target genes that control cell proliferation and differentiation during development (12,13).

  1. Mohan, M. et al. (2010) Nat Rev Cancer 10, 721-8.
  2. Lin, C. et al. (2010) Mol Cell 37, 429-37.
  3. Drexler, H.G. et al. (2004) Leukemia 18, 227-32.
  4. Smith, E. et al. (2011) Genes Dev 25, 661-72.
  5. Shilatifard, A. et al. (1996) Science 271, 1873-6.
  6. Shilatifard, A. et al. (1997) Proc Natl Acad Sci U S A 94, 3639-43.
  7. Miller, T. et al. (2000) J Biol Chem 275, 32052-6.
  8. Lin, C. et al. (2011) Genes Dev 25, 1486-98.
  9. Yokoyama, A. et al. (2010) Cancer Cell 17, 198-212.
  10. Cho, S. et al. (2010) Cell Cycle 9, 1697-705.
  11. Shah, N. and Sukumar, S. (2010) Nat Rev Cancer 10, 361-71.
  12. Mohan, M. et al. (2010) Genes Dev 24, 574-89.
  13. Nguyen, A.T. and Zhang, Y. (2011) Genes Dev 25, 1345-58.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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