Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Ikaros (D6N9Y) Rabbit mAb #14859

sc-398265  

No. Size Price
14859S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14859 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 50-70 Rabbit IgG
IHC-P 1:500
F 1:50
IF-IC 1:100
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Specificity / Sensitivity

Ikaros (D6N9Y) Rabbit mAb recognizes endogenous levels of total Ikaros protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro329 of human Ikaros protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and Jurkat cells (green) using Ikaros (D6N9Y) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ikaros (D6N9Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, detectable expression is restricted exclusively to lymphoid cell lines.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Jurkat cells and either 5 μl of Ikaros (D6N9Y) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human HES1 promoter primers, SimpleChIP® Human HES4 Promoter Primers #7273, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A20 (left), Ramos (middle), and HeLa (right) cells using Ikaros (D6N9Y) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Ikaros (D6N9Y) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma using Ikaros (D6N9Y) Rabbit mAb.

Background

The Ikaros family of zinc-finger DNA-binding proteins belongs to the Kruppel transcription factor superfamily. Ikaros proteins are characterized by the presence of an amino-terminal zinc finger DNA-binding domain and a carboxy-terminal dimerization domain. Members of the Ikaros family include Ikaros, Aiolos, Helios, EOS, and Pegasus (1). All family members can form homodimers and heterodimers with other members of the Ikaros family. Most also contain multiple isoforms that are generated as a result of differential splicing, with some isoforms behaving in a dominant negative manner upon dimerization (2).

Ikaros (IKZF1, LYF1) is the prototypical Ikaros family zinc finger transcription factor, and is expressed abundantly in lymphoid cells. Genetic studies in mice demonstrate that Ikaros is a tumor suppressor that is important for the normal development of B, T, natural killer, and dendritic cells (3,4). Additional studies show that imbalanced expression of different Ikaros isoforms, as well as mutations in the corresponding IKAROS gene, can be associated with a number of hematologic malignancies in humans (2,5,6).

  1. Perdomo, J. et al. (2000) J Biol Chem 275, 38347-54.
  2. Rebollo, A. and Schmitt, C. (2003) Immunol Cell Biol 81, 171-5.
  3. Georgopoulos, K. et al. (1997) Annu Rev Immunol 15, 155-76.
  4. Nichogiannopoulou, A. et al. (1998) Semin Immunol 10, 119-25.
  5. Tonnelle, C. et al. (2002) Leuk Lymphoma 43, 29-35.
  6. Martinelli, G. et al. (2009) J Clin Oncol 27, 5202-7.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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