Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

TRF2 (P449) Antibody #14824

No. Size Price
14824S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14824 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 65 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

TRF2 (P449) Antibody recognizes endogenous levels of total TRF2 protein. This antibody also cross-reacts with an unidentified protein of 135 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro449 of human TRF2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7, COS-7, and HT-29 cells using TRF2 (P449) Antibody.

Background

Telomeres, the linear ends of chromosomes, are organized into T-loops to prevent them from being recognized by the cell as DNA double stranded breaks (DSBs) (1). The telomeric repeat binding factor proteins TRF1 and TRF2 bind to double-stranded telomeres to allow formation of T-loops (2). A large number of proteins involved in the DNA damage response are found physically associated with TRF2 within telomeres (3). Interestingly, TRF2 can transiently localize to DNA damage-induced DSBs, but overexpression of TRF2 prevents ATM-dependent signaling (4). Phosphorylation of TRF2 at Ser323 has been reported in vivo, but no upstream kinase or role has been established for this phosphorylation site (5).

  1. Karlseder, J. et al. (1999) Science 283, 1321-5.
  2. Yoshimura, S.H. et al. (2004) Genes Cells 9, 205-18.
  3. Colgin, L. and Reddel, R. (2004) Curr Biol 14, R901-2.
  4. Bradshaw, P.S. et al. (2005) Nat Genet 37, 193-7.
  5. Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci U S A 101, 12130-5.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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