Cell Signaling Technology

Product Pathways - Protein Stability

OTUD7B/Cezanne-1 Antibody #14817

Cezanne   Cezanne-1   Cezanne1   DUBs   OTUD7B   Ubiquitin   Ubiquitination  

No. Size Price
14817S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
14817 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 98 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

OTUD7B/Cezanne-1 Antibody recognizes endogenous levels of total OTUD7B/Cezanne-1 protein. This antibody does not cross-react with either OTUD7A or OTUD7C/A20

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human OTUD7B/Cezanne-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using OTUD7B/Cezanne-1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human OTUD7B protein (hOTUD7B-Myc/DDK; +), using OTUD7B/Cezanne-1 Antibody.



Immunoprecipitation of OTUD7B/Cezanne-1 from 293T cell extracts using Normal Rabbit IgG #2729 (lane 2) or OTUD7B/Cezanne-1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using OTUD7B/Cezanne-1 Antibody.


Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes and deubiquitinating enzymes respectively (1,2). Deubiquitinating enzymes (DUBS) are categorized into five subfamilies based on catalytic domain structure: USP, UCH, OTU, MJD, and JAMM. The deubiquitinase cellular zinc-finger anti-NF-κB (Cezanne-1, OTUD7B) is an OTU family deubiquitinase that contains amino-terminal catalytic and ubiquitin-associated (UBA) domains, and a carboxy-terminal A20-like zinc finger (A20-ZnF) that is involved in ubiquitin binding (3,4). Research studies demonstrate that Cezanne-1 negatively regulates canonical NF-κB signaling induced by TNF receptor signaling by removing K63-linked ubiquitin chains from the RIP1 adaptor protein (5,6). Cezanne-1 negatively regulates non-canonical NF-κB signaling through the deubiquitination and stabilization of the TRAF3 signal transduction protein (7). Additional research suggests that Cezanne-1 is a breast cancer oncogene as the corresponding OTUD7B gene is amplified in a subset of breast cancers and enhances EGFR signaling through a mechanism involving receptor stabilization (8).

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Mevissen, T.E. et al. (2013) Cell 154, 169-84.
  4. Evans, P.C. et al. (2003) J Biol Chem 278, 23180-6.
  5. Evans, P.C. et al. (2001) Biochem J 357, 617-23.
  6. Enesa, K. et al. (2008) J Biol Chem 283, 7036-45.
  7. Hu, H. et al. (2013) Nature 494, 371-4.
  8. Pareja, F. et al. (2012) Oncogene 31, 4599-608.

Application References

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