Cell Signaling Technology

Product Pathways - MAPK Signaling

RSK1/RSK2/RSK3 (D7A2H) Rabbit mAb #14813

p90RSK   RPS6KA   RPS6KA1   RPS6KA2   RPS6KA3   RSK   RSK1   RSK2   RSK3  

No. Size Price
14813S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14813 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey,Bovine,Dog,Pig, Endogenous 90 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Xenopus, Zebrafish, Guinea Pig,

Specificity / Sensitivity

RSK1/RSK2/RSK3 (D7A2H) Rabbit mAb recognizes endogenous levels of total RSK1, RSK2, and RSK3 proteins. This antibody is predicted to cross-react with RSK4 but has approximately 100-fold greater affinity for RSK1, RSK2, and RSK3. This antibody does not cross-react with other homologous RSK family members, such as human or mouse MSK1 or MSK2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro583 of human RSK1 protein (Pro587 in hRSK2, Pro580 in hRSK3).

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell lines using RSK1/RSK2/RSK3 (D7A2H) Rabbit mAb.

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

  1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
  2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
  3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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