Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

PLK2 (D5R2B) Rabbit mAb #14812

No. Size Price
14812S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14812 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 70 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

PLK2 (D5R2B) Rabbit mAb recognizes endogenous levels of total PLK2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro67 of human PLK2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT 116 cells, untreated (-) or treated with Doxorubicin #5927 (0.5 μM, 24 hr; +), using PLK2 (D5R2B) Rabbit mAb (upper), p53 (7F5) Rabbit mAb #2527 (middle), and β-Actin (13E5) Rabbit mAb #4970 (lower).

IP

IP

Immunoprecipitation of PLK2 from ACHN cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PLK2 (D5R2B) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using PLK2 (D5R2B) Rabbit mAb.

Background

At least five distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3, PLK4/SAK, and the non-catalytic PLK5 protein (1). The p53-induced PLK2 functions in centriole duplication, as well as at spindle and S phase checkpoints (3-5). Research studies show that PLK2 expression is related to chemosensitivity in ovarian cancer. Downregulation of PLK2 expression in chemosensitive ovarian cancer cells is associated with a greater chance of relapse in patients following specific treatment regimens (6). PLK2 can phosphorylate α-synuclein at Ser129, which is a site shown to be involved in diseases of the central nervous system (7,8). Polo-like kinase 2 also phosphorylates GEFs and GAPs, regulating Ras and Rap small GTPase function in neurons (9).

  1. de Cárcer, G. et al. (2011) Cell Cycle 10, 2255-62.
  2. Cizmecioglu, O. et al. (2008) Cell Cycle 7, 3548-55.
  3. Burns, T.F. et al. (2003) Mol Cell Biol 23, 5556-71.
  4. Matthew, E.M. et al. (2007) Cell Cycle 6, 2571-8.
  5. Cizmecioglu, O. et al. (2012) J Cell Sci 125, 981-92.
  6. Syed, N. et al. (2011) Cancer Res 71, 3317-27.
  7. Inglis, K.J. et al. (2009) J Biol Chem 284, 2598-602.
  8. Bergeron, M. et al. (2014) Neuroscience 256, 72-82.
  9. Lee, K.J. et al. (2011) Neuron 69, 957-73.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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