Cell Signaling Technology

Product Pathways - Vesicle Trafficking

VAMP7 (D4D5J) Rabbit mAb #14811

No. Size Price
14811S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14811 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 25 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

VAMP7 (D4D5J) Rabbit mAb recognizes endogenous levels of total VAMP7 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala94 of human VAMP7 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using VAMP7 (D4D5J) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human VAMP7 protein (hVAMP7-Myc/DDK; +), using VAMP7 (D4D5J) Rabbit mAb.

Background

Proteins in the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex are integral membrane proteins involved in vesicle transport and membrane fusion that pair vesicular SNAREs (v-SNAREs) with cognate target SNARE (t-SNARE) proteins (reviewed in 1,2). Vesicle-associated membrane protein 7 (VAMP7), or tetanus neurotoxin-insensitive VAMP (TI-VAMP), is a widely expressed v-SNARE involved in exocytosis of granules and synaptic vesicles in various cell types, membrane remodeling, neurite outgrowth, lysosomal secretion, and autophagosome maturation (3). Activity of VAMP7 can be regulated by c-Src-mediated tyrosine phosphorylation, which activates VAMP7-mediated exocytosis (4). VAMP7 activity can also be regulated through interaction with the guanine nucleotide exchange factor Varp (5,6). Several research studies indicate that VAMP7 plays an important role in neurite outgrowth as well as potential neurological activities, including anxiety (7-9). VAMP7 also appears to have a key role in T-cell activation by facilitating the recruitment of vesicular Lat to the immunological synapse (10). The VAMP7 protein interacts with ATG16L, a component of the ATG5-ATG12 complex, and regulates autophagosome maturation through homotypic fusion of ATG16L1 vesicles (11).

  1. Jena, B.P. (2011) Adv Exp Med Biol 713, 13-32.
  2. Kasai, H. et al. (2012) Physiol Rev 92, 1915-64.
  3. Galli, T. et al. (1998) Mol Biol Cell 9, 1437-48.
  4. Burgo, A. et al. (2013) J Biol Chem 288, 11960-72.
  5. Burgo, A. et al. (2012) Dev Cell 23, 166-80.
  6. Schäfer, I.B. et al. (2012) Nat Struct Mol Biol 19, 1300-9.
  7. Martinez-Arca, S. et al. (2000) J Cell Biol 149, 889-900.
  8. Alberts, P. et al. (2003) Mol Biol Cell 14, 4207-20.
  9. Danglot, L. et al. (2012) J Neurosci 32, 1962-8.
  10. Larghi, P. et al. (2013) Nat Immunol 14, 723-31.
  11. Moreau, K. et al. (2011) Cell 146, 303-17.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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