Cell Signaling Technology

Product Pathways - Protein Stability

NUB1 Antibody #14810

NEDDylation   NUB-1   NUB-1L   NUB1   NUB1L   NY-REN-18   NYREN-18   NYREN18  

No. Size Price
14810S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14810 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 78 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

NUB1 Antibody recognizes endogenous levels of total NUB1 protein. Based upon sequence alignment, this antibody is predicted to detect both NUB1 and NUB1L.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NUB1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NUB1 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, U-2 OS cells express low levels of endogenous NUB1 protein (3).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human NUB1L protein (hNUB1L-Myc/DDK; +), using NUB1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 72hr; +), using NUB1 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Background

Both the NEDD8 ultimate buster 1 (NUB1) and the related NUB1L isoform are interferon-inducible adaptor proteins that negatively regulate ubiquitin-like protein NEDD8 (1,2). NUB1 protein contains an amino terminal ubiquitin-like (UBL) domain and multiple carboxy terminal ubiquitin-associated (UBA) domains. The NUB1L isoform is generated by alternative splicing and contains an extra UBA domain relative to NUB1 (2). Research studies indicate that NUB1 and NUB1L non-covalently bind NEDD8 and facilitate delivery of both NEDD8 monomers and NEDD8 conjugates to the proteasome for degradation (2-5). In addition, NUB1L binds and enhances the proteasomal degradation of the FAT10 ubiquitin-like protein (6). Additional research shows that NUB1 negatively regulates cell proliferation, likely due to inhibition of NEDD8 conjugation to SCF ubiquitin ligases, which leads to inhibition of p27 and cyclin E ubiquitination (3,7). NUB1 has been identified as a putative therapeutic target in Huntington's disease as NUB1 promotes a decrease in levels of mutant HTT protein (8).

  1. Kamitani, T. et al. (2001) J Biol Chem 276, 46655-60.
  2. Tanaka, T. et al. (2003) J Biol Chem 278, 32905-13.
  3. Kito, K. et al. (2001) J Biol Chem 276, 20603-9.
  4. Tanji, K. et al. (2005) Biochem Biophys Res Commun 337, 116-20.
  5. Liu, S. et al. (2013) J Biol Chem 288, 31339-49.
  6. Hipp, M.S. et al. (2004) J Biol Chem 279, 16503-10.
  7. Hosono, T. et al. (2010) Br J Cancer 102, 873-82.
  8. Lu, B. et al. (2013) Nat Neurosci 16, 562-70.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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