Cell Signaling Technology

Product Pathways - Protein Stability

APC10 Antibody #14807

Anaphase Promotinfg Complex   APC-10   APC/C   APC10   DOC-1   DOC1   E3 Ubiquitin Ligases  

No. Size Price
14807S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14807 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 24 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Chicken, Bovine, Pig,

Specificity / Sensitivity

APC10 Antibody recognizes endogenous levels of total APC10 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human APC10 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using APC10 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human APC10 protein (hAPC10-Myc/DDK; +), using APC10 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells expressing either non-targeting shRNA (shNT) or shAPC10 as indicated (+), using APC10 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).


Cell proliferation in all eukaryotic cells depends strictly upon the E3 ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. APC/C performs its various functions by promoting the assembly of polyubiquitin chains on substrate proteins, which targets these proteins for degradation by the 26S proteasome (1,2). In humans, twelve different APC/C subunits have been identified. Like all E3 enzymes, APC/C utilizes ubiquitin residues that have been activated by E1 enzymes and then transferred to E2 enzymes. Indeed, APC/C has been shown to interact with UBE2S and UBE2C E2 enzymes, in part, via the RING-finger domain-containing subunit, APC11 (3-5). APC/C activity is also strictly dependent upon its association with multiple cofactors. For example, the related proteins, cell division control protein 20 homolog (CDC20) and Cdh1/FZR1, participate in the recognition of APC/C substrates by interacting with specific recognition elements in these substrates (6), called D-boxes (7) and KEN-boxes (8).

Anaphase-promoting complex subunit 10 (APC10, DOC1) is a highly conserved, core component of the anaphase promoting complex/cyclosome (9,10). Research studies indicate that APC10 participates in substrate recognition by the APC/C (3,11,12).

  1. Qiao, X. et al. (2010) Cell Cycle 9, 3904-12.
  2. Harper, J.W. et al. (2002) Genes Dev 16, 2179-206.
  3. Chang, L. et al. (2014) Nature 513, 388-93.
  4. Carroll, C.W. and Morgan, D.O. (2002) Nat Cell Biol 4, 880-7.
  5. Gmachl, M. et al. (2000) Proc Natl Acad Sci U S A 97, 8973-8.
  6. Leverson, J.D. et al. (2000) Mol Biol Cell 11, 2315-25.
  7. Kraft, C. et al. (2005) Mol Cell 18, 543-53.
  8. Glotzer, M. et al. (1991) Nature 349, 132-8.
  9. Pfleger, C.M. and Kirschner, M.W. (2000) Genes Dev 14, 655-65.
  10. Kurasawa, Y. and Todokoro, K. (1999) Oncogene 18, 5131-7.
  11. Grossberger, R. et al. (1999) J Biol Chem 274, 14500-7.
  12. Carroll, C.W. et al. (2005) Curr Biol 15, 11-8.
  13. Passmore, L.A. et al. (2003) EMBO J 22, 786-96.

Application References

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