Cell Signaling Technology

Product Pathways - Metabolism

LSR (D3E3N) XP® Rabbit mAb #14804

Lipolysis stimulated lipoprotein receptor   LISCH   LISCH7   LSR  

No. Size Price
14804S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
14804T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
14804 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 65 Rabbit IgG
IP 1:50
IHC-P 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

LSR (D3E3N) XP® Rabbit mAb recognizes endogenous levels of total LSR protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly449 of human LSR protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded DLD-1 (left) and HeLa (right) cell pellets using LSR (D3E3N) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using LSR (D3E3N) XP® Rabbit mAb (upper) and β-Actin (D6A8) #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using LSR (D3E3N) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using LSR (D3E3N) XP® Rabbit mAb.

Background

The lipolysis-stimulated lipoprotein receptor (LSR, LISCH) is an immunoglobulin superfamily member and single pass transmembrane protein that binds the apolipoprotein B (ApoB) and apolipoprotein E (ApoE) lipoproteins (1). LSR is responsible for the cellular uptake of triacylglyceride-rich lipoproteins and supports lipid distribution between the liver and peripheral tissues (1,2). The LSR protein is expressed at the cell membrane as a heterodimer consisting of α and β subunits, which are produced as alternative splice variants from a single gene (3). Research studies suggest that LSR acts as the host cell surface receptor for multiple Clostridium toxins (4) and participates in the formation of tricellular tight junctions in epithelial cells (5). Additional studies demonstrate that LSR expression is up-regulated in several cancer types, including breast, bladder, and colorectal cancer, which could lead to pro-tumorigenic changes in metabolism (6-8).

  1. Bihain, B.E. and Yen, F.T. (1992) Biochemistry 31, 4628-36.
  2. Yen, F.T. et al. (1994) Biochemistry 33, 1172-80.
  3. Yen, F.T. et al. (1999) J Biol Chem 274, 13390-8.
  4. Papatheodorou, P. et al. (2012) Infect Immun 80, 1418-23.
  5. Masuda, S. et al. (2011) J Cell Sci 124, 548-55.
  6. García, J.M. et al. (2007) Clin Cancer Res 13, 6351-8.
  7. Herbsleb, M. et al. (2008) BMC Med Genomics 1, 31.
  8. Reaves, D.K. et al. (2014) PLoS One 9, e91747.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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