Cell Signaling Technology

Product Pathways - Adhesion

Integrin β4 (D8P6C) XP® Rabbit mAb #14803

beta4   CD104   GP150   integrin   integrin beta4   ITGB4  

No. Size Price
14803S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
14803T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
14803 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 210 Rabbit IgG
IHC-P 1:200
F 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,

Specificity / Sensitivity

Integrin β4 (D8P6C) XP® Rabbit mAb recognizes endogenous levels of total integrin β4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human integrin β4 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HT-29 (left) and LNCaP (right) cell pellets using Integrin β4 (D8P6C) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β4 (D8P6C) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Integrin β4 (D8P6C) XP® Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29, LNCaP, and A-204 cells using Integrin β4 (D8P6C) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of A-204 cells (blue) and A-431 cells (green) using Integrin β4 (D8P6C) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2).

Integrin β4 pairs with integrin α6 on the cell surface membrane to form the integrin α6β4 heterodimer, an important laminin receptor that is essential for hemidesmosome formation and the support of stable adhesions between basal epithelial cells and the basement membrane (4,5). Integrin β4 is an important component in several growth factor induced signaling pathways that are involved in tumorigenesis and invasive cell growth (6,7).

  1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
  2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
  3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.
  4. Schaapveld, R.Q. et al. (1998) J Cell Biol 142, 271-84.
  5. Litjens, S.H. et al. (2006) Trends Cell Biol 16, 376-83.
  6. Guo, W. et al. (2006) Cell 126, 489-502.
  7. Bertotti, A. et al. (2006) J Cell Biol 175, 993-1003.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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