Cell Signaling Technology

Product Pathways - Apoptosis

Bax (D3R2M) Rabbit mAb (Rodent Preferred) #14796

sc-23959   sc-526   sc-7480  

No. Size Price
14796S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14796 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse,Rat, Endogenous 20 Rabbit IgG
IP 1:50
IHC-P 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Bax (D3R2M) Rabbit mAb (Rodent Preferred) recognizes endogenous levels of total Bax protein in rodent samples. It can detect human Bax protein by western blot, but not by other applications.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu70 of mouse Bax protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Bax (D3R2M) Rabbit mAb (Rodent Preferred).

Western Blotting

Western Blotting

Western blot analysis of extracts from Bax WT MEF and Bax -/- MEF cells using Bax (D3R2M) Rabbit mAb (Rodent Preferred) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). MEF cells are courtesy of Dr. Junying Yuan, of Harvard Medical School, Boston, MA.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Bax WT MEF (left) and Bax -/- MEF (right) cell pellets using Bax (D3R2M) Rabbit mAb (Rodent Preferred). MEF cells are courtesy of Dr. Junying Yuan, of Harvard Medical School, Boston, MA.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded rat spleen using Bax (D3R2M) Rabbit mAb (Rodent Preferred).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse kidney using Bax (D3R2M) Rabbit mAb (Rodent Preferred).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung using Bax (D3R2M) Rabbit mAb (Rodent Preferred).

Background

Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (3,4).

  1. Wei, M.C. et al. (2001) Science 292, 727-30.
  2. Jürgensmeier, J.M. et al. (1998) Proc Natl Acad Sci U S A 95, 4997-5002.
  3. Narita, M. et al. (1998) Proc Natl Acad Sci U S A 95, 14681-6.
  4. Marzo, I. et al. (1998) Science 281, 2027-31.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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