Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

DTX3L (D5F2J) Rabbit mAb #14795

No. Size Price
14795S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14795 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 84 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

DTX3L (D5F2J) Rabbit mAb recognizes endogenous levels of total DTX3L protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val22 of human DTX3L protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431, MCF7, and HT-29 cells using DTX3L (D5F2J) Rabbit mAb.

IP

IP

Immunoprecipitation of DTX3L from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is DTX3L (D5F2J) Rabbit mAb. Western blot analysis was performed using DTX3L (D5F2J) Rabbit mAb.

Background

The E3 ubiquitin-protein ligase DTX3L (BBAP) is a deltex (DTX) family ligase that binds B aggressive lymphoma 1 (BAL1) to mediate the monoubiquitination of histone H4 Lys91 in response to DNA damage (1,2). The early recruitment of poly-ADP-ribose polymerase 1 (PARP1) protein to sites of DNA damage leads to poly-ADP-ribosylation (PARylation) of multiple target proteins. This results in the subsequent recruitment of DTX3L/BAL1 through binding of the BAL macrodomain to PARylated proteins. DTX3L/BAL1 then mediates the monoubiquitination of histone H4 Lys91 that is required for subsequent methylation of histone H4 Lys20 and recruitment of p53-binding protein 1 (1-3). DTX3L and BAL1 are highly expressed in malignant B-cell lymphomas and act to confer chemotherapy-resistance by protecting cells from DNA damaging agents (4).

  1. Takeyama, K. et al. (2003) J Biol Chem 278, 21930-7.
  2. Yan, Q. et al. (2009) Mol Cell 36, 110-20.
  3. Yan, Q. et al. (2013) Mol Cell Biol 33, 845-57.
  4. Juszczynski, P. et al. (2006) Mol Cell Biol 26, 5348-59.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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