Product Pathways - Cell Cycle / Checkpoint
DTX3L (D5F2J) Rabbit mAb #14795
|14795S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|14795||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
DTX3L (D5F2J) Rabbit mAb recognizes endogenous levels of total DTX3L protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val22 of human DTX3L protein.
Western blot analysis of extracts from A-431, MCF7, and HT-29 cells using DTX3L (D5F2J) Rabbit mAb.
The E3 ubiquitin-protein ligase DTX3L (BBAP) is a deltex (DTX) family ligase that binds B aggressive lymphoma 1 (BAL1) to mediate the monoubiquitination of histone H4 Lys91 in response to DNA damage (1,2). The early recruitment of poly-ADP-ribose polymerase 1 (PARP1) protein to sites of DNA damage leads to poly-ADP-ribosylation (PARylation) of multiple target proteins. This results in the subsequent recruitment of DTX3L/BAL1 through binding of the BAL macrodomain to PARylated proteins. DTX3L/BAL1 then mediates the monoubiquitination of histone H4 Lys91 that is required for subsequent methylation of histone H4 Lys20 and recruitment of p53-binding protein 1 (1-3). DTX3L and BAL1 are highly expressed in malignant B-cell lymphomas and act to confer chemotherapy-resistance by protecting cells from DNA damaging agents (4).
- Takeyama, K. et al. (2003) J Biol Chem 278, 21930-7.
- Yan, Q. et al. (2009) Mol Cell 36, 110-20.
- Yan, Q. et al. (2013) Mol Cell Biol 33, 845-57.
- Juszczynski, P. et al. (2006) Mol Cell Biol 26, 5348-59.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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