Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

iNOS (D6B6S) Rabbit mAb (PE Conjugate) #14792

No. Size Price
14792S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
14792 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Mouse, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

iNOS (D6B6S) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total iNOS protein. This antibody does not cross-react with other NOS proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1133 of mouse iNOS protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated iNOS (D6B6S) Rabbit mAb #13120.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of RAW 309 Cr.1 cells, untreated (blue) or treated with LPS #14011 (1 μg/ml, 16 hr; green), using iNOS (D6B6S) Rabbit mAb (PE Conjugate).

Background

Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).

  1. Tsutsui, M. (2004) J Atheroscler Thromb 11, 41-48.
  2. Son, H. et al. (1996) Cell 87, 1015-10123.
  3. Hawkins, R.D. (1996) Neuron 16, 465-467.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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