Cell Signaling Technology

Product Pathways - Metabolism

CBS (D8F2P) Rabbit mAb #14782

No. Size Price
14782S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14782 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 61 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CBS (D8F2P) Rabbit mAb recognizes endogenous levels of total CBS protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu220 of human CBS protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CBS (D8F2P) Rabbit mAb.

Background

Cystathionine beta-synthase (CBS) is a key enzyme involved in sulfur amino acid metabolism because it catalyzes the formation of cystathionine from serine and homocysteine (1,2). The CBS protein contains a heme-binding domain that modulates enzyme activity by sensing redox changes or carbon monoxide binding (1). S-adenosylmethionine binds the carboxyl-terminal CBS domain to allosterically regulate CBS catalytic activity (3,4). In addition to catalyzing cystathionine formation, CBS also catalyzes the generation of hydrogen sulfide, a neuromodulator in the brain, through alternative reactions (5,6). Mutations in the corresponding CBS gene result in homocystinuria, an autosomal recessive disorder characterized by abnormal sulfur metabolism, mental retardation, eye anomalies, and vascular disease (7).

  1. Banerjee, R. and Zou, C.G. (2005) Arch Biochem Biophys 433, 144-56.
  2. Jhee, K.H. and Kruger, W.D. (2005) Antioxid Redox Signal 7, 813-22.
  3. Ereño-Orbea, J. et al. (2014) Proc Natl Acad Sci U S A 111, E3845-52.
  4. McCorvie, T.J. et al. (2014) J Biol Chem 289, 36018-30.
  5. Kimura, H. (2010) Antioxid Redox Signal 12, 1111-23.
  6. Zhang, Y. et al. (2013) Mol Cell Biol 33, 1104-13.
  7. Kraus, J.P. et al. (1999) Hum Mutat 13, 362-75.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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