Cell Signaling Technology

Product Pathways - Protein Stability

SYVN1 (D3O2A) Rabbit mAb #14773

E3 Ubiquitin Ligases   ERAD   HRD-1   HRD1   SYVN-1   SYVN1  

No. Size Price
14773S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14773 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 75 Rabbit IgG
IP 1:200
IF-IC 1:6400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

SYVN1 (D3O2A) Rabbit mAb recognizes endogenous levels of total SYVN1 protein. This antibody does not cross-react with AMFR protein, but may cross-react with an unidentified protein of 60 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SYVN1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SYVN1 (D3O2A) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human AMFR protein (hAMFR-Myc/DDK; +) or Myc/DDK-tagged full-length human SYVN1 protein (hSYVN1-Myc/DDK; +), using SYVN1 (D3O2A) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower).

IP

IP

Immunoprecipitation of SYVN1 from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SYVN1 (D3O2A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SYVN1 (D3O2A) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Thapsigargin #12758 (1 μM, 16 hr; +) or Tunicamycin #12819 (5 μg/ml, 16 hr; +), using SYVN1 (D3O2A) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

IF-IC

IF-IC

Confocal immunofluorescent analysis of SH-SY5Y cells, untreated (left) or treated with Tunicamycin #12819 (10 μg/mL for 16 hrs; center), and HeLa cells (right) using SYVN1 (D3O2A) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y cells, untreated (-) or treated with Tunicamycin #12819 (10ug/mL, 16h; +), using SYVN1 (D3O2A) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Background

Synoviolin-1 (SYVN1/HRD1) is a RING-type E3 ubiquitin-protein ligase and major component of the endoplasmic reticulum (ER) quality control system that is involved in the ubiquitin-dependent degradation of misfolded proteins (1). SYVN1 is a multispanning ER membrane protein whose expression is upregulated at the protein level under conditions that promote ER stress (1-4). Research studies have shown that SYVN1 is an anti-apoptotic factor that is implicated in the pathogenesis of arthropathy by promoting synovial hyperplasia (5). Furthermore, gene-targeting studies have demonstrated that SYVN1 expression is indispensable for embryogenesis (6).

  1. Kikkert, M. et al. (2004) J Biol Chem 279, 3525-34.
  2. Kaneko, M. et al. (2007) FEBS Lett 581, 5355-60.
  3. Yamamoto, K. et al. (2008) J Biochem 144, 477-86.
  4. Nadav, E. et al. (2003) Biochem Biophys Res Commun 303, 91-7.
  5. Amano, T. et al. (2003) Genes Dev 17, 2436-49.
  6. Yagishita, N. et al. (2005) J Biol Chem 280, 7909-16.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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