Cell Signaling Technology

Product Pathways - Adhesion

CEACAM1 (D1P4T) Rabbit mAb #14771

Carcinoembryonic antigen  

No. Size Price
14771S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
14771 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 160 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

CEACAM1 (D1P4T) Rabbit mAb recognizes endogenous levels of total CEACAM1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala460 of human CEACAM1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CEACAM1 (D1P4T) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of CEACAM1 from SK-MEL-5 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CEACAM1 (D1P4T) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CEACAM1 (D1P4T) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.

Background

CEACAM1 (also known as C-CAM and CD66a) is a member of CEA-related cell-adhesion molecule (CEACAM) subfamily of the carcinoembryonic antigen (CEA) family (1). CEACAM1 is expressed by certain epithelial, endothelial, lymphoid, and myeloid cells. Human CEACAM1 has many different splice variants; the abundance of CEACAM1 and the relative ratio of the different isoforms varies markedly among cell types and may be regulated in a context-dependent fashion. The isoforms with long (L) and short (S) cytoplasmic tails have different signaling properties. Notably, L isoforms contain a functional ITIM (immunoreceptor tyrosine-based inhibitory motif) and several serine and threonine residues that could serve as potential phosphorylation targets. The extracellular domain of CEACAM1 is heavily glycosylated, making its apparent molecular weight during electrophoresis much larger than its predicted size (57.6 kDa) (2). CEACAM1 mediates intercellular adhesion through homo- and heterophilic interaction with other members of the CEACAM family. Studies indicate that CEACAM1 plays important roles in angiogenesis, neovascularization, insulin signaling, T cell signaling, and tumorigenesis (3-8). In addition, CEACAM1 can function as a receptor for several microbial pathogens (9,10).

  1. Kuespert, K. et al. (2006) Curr Opin Cell Biol 18, 565-71.
  2. Gray-Owen, S.D. and Blumberg, R.S. (2006) Nat Rev Immunol 6, 433-46.
  3. Horst, A.K. et al. (2006) J Clin Invest 116, 1596-605.
  4. Ergün, S. et al. (2000) Mol Cell 5, 311-20.
  5. Kammerer, R. et al. (2001) J Immunol 166, 6537-44.
  6. Poy, M.N. et al. (2002) Nat Genet 30, 270-6.
  7. Hsieh, J.T. et al. (1995) Cancer Res 55, 190-7.
  8. Leung, N. et al. (2006) Oncogene 25, 5527-36.
  9. Hemmila, E. et al. (2004) J Virol 78, 10156-65.
  10. Voges, M. et al. (2010) BMC Microbiol 10, 117.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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